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Nanomagnetic Gene Transfection for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts
Institute for Science & Technology in Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent ST4 7QB, UK
Crayton Pruitt Family Department of Biomedical Engineering and Department of Materials Science & Engineering, University of Florida, P.O. Box 116400, Gainesville, FL 32611, USA
Institute for Cell Engineering and Regenerative Medicine (ICERM), University of Florida, P.O. Box 116131, Gainesville, FL 32611, USA
* Author to whom correspondence should be addressed.
Received: 15 November 2012; in revised form: 8 January 2013 / Accepted: 11 January 2013 / Published: 18 January 2013
Abstract: The objective of this work was to examine the potential of oscillating nanomagnetic gene transfection systems (magnefect-nano™) for improving the transfection efficiency of NIH3T3 mouse embryonic fibroblasts (MEFs) in comparison to other non-viral transfection techniques—static magnetofection™ and the cationic lipid agent, Lipofectamine 2000™. Magnetic nanoparticles (MNPs) associated with the plasmid coding for green fluorescent protein (GFP) were used to transfect NIH3T3 cells. The magnefect-nano system was evaluated for transfection efficiency, and any potential associated effects on cell viability were investigated. MNPs associated with the plasmid coding for GFP were efficiently delivered into NIH3T3 cells, and the magnefect-nano system significantly enhanced overall transfection efficiency in comparison to lipid-mediated gene delivery. MNP dosage used in this work was not found to affect the cell viability and/or morphology of the cells. Non-viral transfection using MNPs and the magnefect-nano system can be used to transfect NIH3T3 cells and direct reporter gene delivery, highlighting the wide potential of nanomagnetic gene transfection in gene therapy.
Keywords: non-viral gene transfection; nanomagnetic gene transfection; DNA; magnetic nanoparticles; magnetic field
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MDPI and ACS Style
Fouriki, A.; Dobson, J. Nanomagnetic Gene Transfection for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts. Materials 2013, 6, 255-264.
Fouriki A, Dobson J. Nanomagnetic Gene Transfection for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts. Materials. 2013; 6(1):255-264.
Fouriki, Angeliki; Dobson, Jon. 2013. "Nanomagnetic Gene Transfection for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts." Materials 6, no. 1: 255-264.