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Materials 2011, 4(4), 703-715; doi:10.3390/ma4040703
Article

Efficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings

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1 Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China 2 Center of Novel Functional Molecules and Institute of Molecular Functional Materials, Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China 3 Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
* Author to whom correspondence should be addressed.
Received: 7 March 2011 / Revised: 29 March 2011 / Accepted: 2 April 2011 / Published: 7 April 2011
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Abstract

We herein report a comparative study of mesenchymal stem cell (MSC) labeling using spherical superparamagnetic iron oxide (SPIO) nanoparticles containing different coatings, namely, organosilica, dextran, and poly(ethylene glycol) (PEG). These nanomaterials possess a similar SPIO core size of 6–7 nm. Together with their coatings, the overall sizes are 10–15 nm for all SPIO@SiO2, SPIO@dextran, and SPIO@PEG nanoparticles. These nanoparticles were investigated for their efficacies to be uptaken by rabbit bone marrow-derived MSCs without any transfecting agent. Experimentally, both SPIO@SiO2 and SPIO@PEG nanoparticles could be successfully uptaken by MSCs while the SPIO@dextran nanoparticles demonstrated limited labeling efficiency. The labeling durability of SPIO@SiO2 and SPIO@PEG nanoparticles in MSCs after three weeks of culture were compared by Prussian blue staining tests. SPIO@SiO2 nanoparticles demonstrated more blue staining than SPIO@PEG nanoparticles, rendering them better materials for MSCs labeling by direct uptake when durable intracellullar retention of SPIO is desired.
Keywords: magnetic nanoparticle; surface modification; cell labeling magnetic nanoparticle; surface modification; cell labeling
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Wang, Y.-X.; Quercy-Jouvet, T.; Wang, H.-H.; Li, A.-W.; Chak, C.-P.; Xuan, S.; Shi, L.; Wang, D.-F.; Lee, S.-F.; Leung, P.-C.; Lau, C.B.S.; Fung, K.-P.; Leung, K.-F. Efficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings. Materials 2011, 4, 703-715.

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