Materials 2011, 4(4), 703-715; doi:10.3390/ma4040703
Article

Efficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings

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Received: 7 March 2011; in revised form: 29 March 2011 / Accepted: 2 April 2011 / Published: 7 April 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We herein report a comparative study of mesenchymal stem cell (MSC) labeling using spherical superparamagnetic iron oxide (SPIO) nanoparticles containing different coatings, namely, organosilica, dextran, and poly(ethylene glycol) (PEG). These nanomaterials possess a similar SPIO core size of 6–7 nm. Together with their coatings, the overall sizes are 10–15 nm for all SPIO@SiO2, SPIO@dextran, and SPIO@PEG nanoparticles. These nanoparticles were investigated for their efficacies to be uptaken by rabbit bone marrow-derived MSCs without any transfecting agent. Experimentally, both SPIO@SiO2 and SPIO@PEG nanoparticles could be successfully uptaken by MSCs while the SPIO@dextran nanoparticles demonstrated limited labeling efficiency. The labeling durability of SPIO@SiO2 and SPIO@PEG nanoparticles in MSCs after three weeks of culture were compared by Prussian blue staining tests. SPIO@SiO2 nanoparticles demonstrated more blue staining than SPIO@PEG nanoparticles, rendering them better materials for MSCs labeling by direct uptake when durable intracellullar retention of SPIO is desired.
Keywords: magnetic nanoparticle; surface modification; cell labeling
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MDPI and ACS Style

Wang, Y.-X.; Quercy-Jouvet, T.; Wang, H.-H.; Li, A.-W.; Chak, C.-P.; Xuan, S.; Shi, L.; Wang, D.-F.; Lee, S.-F.; Leung, P.-C.; Lau, C.B.S.; Fung, K.-P.; Leung, K.-F. Efficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings. Materials 2011, 4, 703-715.

AMA Style

Wang Y-X, Quercy-Jouvet T, Wang H-H, Li A-W, Chak C-P, Xuan S, Shi L, Wang D-F, Lee S-F, Leung P-C, Lau CBS, Fung K-P, Leung K-F. Efficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings. Materials. 2011; 4(4):703-715.

Chicago/Turabian Style

Wang, Yi-Xiang J.; Quercy-Jouvet, Thibault; Wang, Hao-Hao; Li, Ak-Wai; Chak, Chun-Pong; Xuan, Shouhu; Shi, Lin; Wang, De-Feng; Lee, Siu-Fung; Leung, Ping-Chung; Lau, Clara B. S.; Fung, Kwok-Pui; Leung, Ken Cham-Fai. 2011. "Efficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings." Materials 4, no. 4: 703-715.

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