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Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups
Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
* Author to whom correspondence should be addressed.
Received: 2 February 2010; in revised form: 2 March 2010 / Accepted: 25 March 2010 / Published: 26 March 2010
Abstract: The objective of this study is to develop an easy and simple diffusion-controlled fabrication technique to generate the concentration gradient of biomolecules in hydrogels. Polyacrylamide (PAAm) hydrogels with a concentration gradient of type I collagen were prepared to evaluate the cell adhesion. The PAAm hydrogel was exposed to a gradient concentration of sodium hydroxide (NaOH) solution at 52 °C to generate that of carboxyl groups in the hydrogel. The carboxyl groups generated were chemically coupled with the amino groups of type I collagen to prepare the hydrogel with a concentration gradient of collagen immobilized. The attachment of L929 fibroblasts was evaluated for the collagen-immobilized hydrogel prepared. The amount gradient of carboxyl groups in the hydrogel increased with an increase in the NaOH concentration while the carboxyl groups gradient enabled to generate a gradient of collagen immobilized in the hydrogel. On the other hand, the number of fibroblasts adhered depended on the amount of collagen immobilized. These findings indicate that the adhesion behavior of cells is modified by the concentration gradient of biomolecule in the three-dimensional scaffold of cells.
Keywords: gradient; diffusion-controlled hydrolysis; collagen; cell attachment; hydrogel
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MDPI and ACS Style
Yamamoto, M.; Yanase, K.; Tabata, Y. Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups. Materials 2010, 3, 2393-2404.
Yamamoto M, Yanase K, Tabata Y. Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups. Materials. 2010; 3(4):2393-2404.
Yamamoto, Masaya; Yanase, Kaoru; Tabata, Yasuhiko. 2010. "Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups." Materials 3, no. 4: 2393-2404.