Next Article in Journal
Ultra-High Molecular Weight Polyethylene: Influence of the Chemical, Physical and Mechanical Properties on the Wear Behavior. A Review
Previous Article in Journal
A Highly-Sensitive Picric Acid Chemical Sensor Based on ZnO Nanopeanuts
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Materials 2017, 10(7), 793; doi:10.3390/ma10070793

Cultured Human Fibroblast Biostimulation Using a 940 nm Diode Laser

1
Biomedical Group (Bio277), Department of Nursing, Faculty of Nursing, University of Granada Campus from Melilla, C/Santander, 1, Melilla 58071, Spain
2
Instituto de Investigación Biosanitaria ibs.GRANADA, University of Granada, C/Doctor Azpitarte 4, planta, Granada 18012, Spain
3
Biomedical Group (Bio277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda de la Ilustración 60, Granada 18016, Spain
4
Biomedical Group (Bio277), Department of Stomatology, School of Dentistry, University of Granada, Campus de Cartuja s/n, Granada 18071, Spain
5
Institute of Neuroscience, University of Granada, Granada Health-Science Technology Park, Armilla, Granada 18100, Spain
*
Author to whom correspondence should be addressed.
Received: 5 June 2017 / Revised: 23 June 2017 / Accepted: 7 July 2017 / Published: 13 July 2017
View Full-Text   |   Download PDF [4589 KB, uploaded 13 July 2017]   |  

Abstract

Background: Fibroblasts are the main cells involved in regeneration during wound healing. The objective was to determine the effect of 940 nm diode laser on cultured human fibroblasts using different irradiation regimens. Methods: The CCD-1064Sk human epithelial fibroblast cell line was treated with a 940 nm diode laser at different energy doses (power: 0.2–1 W and energy density: 1–7 J/cm2) using different transmission modes (continuous or pulsed). The effect on cell growth at 24 and 72 h post-treatment was examined by measuring the proliferative capacity, the impact on the cell cycle, and the effect on cell differentiation. Results: fibroblast proliferative capacity was increased at 24 and 72 h post-treatment as a function of the energy dose. The greatest increase was observed with a power of 0.2 or 0.5 W and energy density between 1 and 4 J/cm2; no difference was observed between continuous and pulsed modes. There were no significant differences in cell cycle between treated groups and controls. α-actin expression was increased by treatment, indicating enhanced cell differentiation. Conclusion: The 940 nm diode laser has biostimulating effects on fibroblasts, stimulating proliferative capacity and cell differentiation without altering the cell cycle. Further researches are necessary to explore its potential clinical usefulness in wound healing. View Full-Text
Keywords: wound healing; fibroblasts; diode laser; biostimulation; cell culture wound healing; fibroblasts; diode laser; biostimulation; cell culture
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Illescas-Montes, R.; Melguizo-Rodríguez, L.; Manzano-Moreno, F.J.; García-Martínez, O.; Ruiz, C.; Ramos-Torrecillas, J. Cultured Human Fibroblast Biostimulation Using a 940 nm Diode Laser. Materials 2017, 10, 793.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top