Characterization and In Vitro and In Vivo Assessment of a Novel Cellulose Acetate-Coated Mg-Based Alloy for Orthopedic Applications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials Synthesis and Characterization
2.1.1. Alloy Synthesis, Thermo-Mechanical Processing, Microstructural and Mechanical Characterization
2.1.2. Coating Formulation
2.1.3. Biomaterials Characterization
2.2. In Vitro Cellular Response
2.2.1. Preparation of Extracts of the Biomaterials
2.2.2. Cell Culture
2.2.3. Quantitative and Qualitative Assessment of Cellular Survival and Cell Proliferation
2.2.4. Fluorescence Microscopic Evaluation of Cell Adhesion and Morphology
2.2.5. Determination of Intracellular Alkaline Phosphatase Activity
2.2.6. Quantification of Osteopontin Secretion
2.2.7. Quantitative Assessment of Extracellular Matrix Mineralization
2.3. In Vivo Animal Studies
2.3.1. Implant Preparation and Surgery
2.3.2. Histological Examination
2.4. Statistical Analysis
3. Results
3.1. Alloy’s Microstructural and Mechanical Characterization
3.2. Characterization of Uncoated and Coated Mg-Based Alloy Samples
3.3. In Vitro Behavior of Osteoblast-Like Cells
3.3.1. Cell Viability/Proliferation and Morphological Features
3.3.2. The Function of MC3T3-E1 Pre-Osteoblasts
3.4. In Vivo Biocompatibility of the Mg-Based Biomaterials
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample | Ecorr (V) | icorr (µA/cm2) | −βc (mV) | βa (mV) | Rp (kΩxcm2) | Pe (%) |
---|---|---|---|---|---|---|
uncoated Mg alloy | −1.85 | 497.96 | 422.92 | 475.14 | 0.195 | - |
Mg-CA | −1.60 | 4.88 | 614.56 | 391.57 | 21.28 | 99.02 |
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Neacsu, P.; Staras, A.I.; Voicu, S.I.; Ionascu, I.; Soare, T.; Uzun, S.; Cojocaru, V.D.; Pandele, A.M.; Croitoru, S.M.; Miculescu, F.; et al. Characterization and In Vitro and In Vivo Assessment of a Novel Cellulose Acetate-Coated Mg-Based Alloy for Orthopedic Applications. Materials 2017, 10, 686. https://doi.org/10.3390/ma10070686
Neacsu P, Staras AI, Voicu SI, Ionascu I, Soare T, Uzun S, Cojocaru VD, Pandele AM, Croitoru SM, Miculescu F, et al. Characterization and In Vitro and In Vivo Assessment of a Novel Cellulose Acetate-Coated Mg-Based Alloy for Orthopedic Applications. Materials. 2017; 10(7):686. https://doi.org/10.3390/ma10070686
Chicago/Turabian StyleNeacsu, Patricia, Adela Ioana Staras, Stefan Ioan Voicu, Iuliana Ionascu, Teodoru Soare, Seralp Uzun, Vasile Danut Cojocaru, Andreea Madalina Pandele, Sorin Mihai Croitoru, Florin Miculescu, and et al. 2017. "Characterization and In Vitro and In Vivo Assessment of a Novel Cellulose Acetate-Coated Mg-Based Alloy for Orthopedic Applications" Materials 10, no. 7: 686. https://doi.org/10.3390/ma10070686