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Bioethanol Production by Carbohydrate-Enriched Biomass of Arthrospira (Spirulina) platensis
Department of Natural Resources Management and Agricultural Engineering, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece
Department of Environmental Engineering, Building 113, Technical University of Denmark, Lyngby 2800, Denmark
Biotechnology and Industrial Fermentations Lab, Faculty of Food Science and Nutrition, Technological Educational Institution of Athens, Ag. Spyridon Street, Egaleo 12210, Athens, Greece
* Author to whom correspondence should be addressed.
Received: 19 June 2013; in revised form: 7 July 2013 / Accepted: 23 July 2013 / Published: 6 August 2013
Abstract: In the present study the potential of bioethanol production using carbohydrate-enriched biomass of the cyanobacterium Arthrospira platensis was studied. For the saccharification of the carbohydrate-enriched biomass, four acids (H2SO4, HNO3, HCl and H3PO4) were investigated. Each acid were used at four concentrations, 2.5 N, 1 N, 0.5 N and 0.25 N, and for each acid concentration the saccharification was conducted under four temperatures (40 °C, 60 °C, 80 °C and 100 °C). Higher acid concentrations gave in general higher reducing sugars (RS) yields (%, gRS/gTotal sugars) with higher rates, while the increase in temperature lead to higher rates at lower acid concentration. The hydrolysates then were used as substrate for ethanolic fermentation by a salt stress-adapted Saccharomyces cerevisiae strain. The bioethanol yield (%, gEtOH/gBiomass) was significantly affected by the acid concentration used for the saccharification of the carbohydrates. The highest bioethanol yields of 16.32% ± 0.90% (gEtOH/gBiomass) and 16.27% ± 0.97% (gEtOH/gBiomass) were obtained in hydrolysates produced with HNO3 0.5 N and H2SO4 0.5 N, respectively.
Keywords: Arthrospira (Spirulina); bioethanol; fermentation; microalgae; pretreatment; acid hydrolysis; thermal hydrolysis
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Markou, G.; Angelidaki, I.; Nerantzis, E.; Georgakakis, D. Bioethanol Production by Carbohydrate-Enriched Biomass of Arthrospira (Spirulina) platensis. Energies 2013, 6, 3937-3950.
Markou G, Angelidaki I, Nerantzis E, Georgakakis D. Bioethanol Production by Carbohydrate-Enriched Biomass of Arthrospira (Spirulina) platensis. Energies. 2013; 6(8):3937-3950.
Markou, Giorgos; Angelidaki, Irini; Nerantzis, Elias; Georgakakis, Dimitris. 2013. "Bioethanol Production by Carbohydrate-Enriched Biomass of Arthrospira (Spirulina) platensis." Energies 6, no. 8: 3937-3950.