Next Article in Journal
Probabilistic Approach to Optimizing Active and Reactive Power Flow in Wind Farms Considering Wake Effects
Next Article in Special Issue
Microalgae for Biofuels and Animal Feeds
Previous Article in Journal / Special Issue
Microalgal Species Selection for Biodiesel Production Based on Fuel Properties Derived from Fatty Acid Profiles
Article Menu

Export Article

Open AccessArticle
Energies 2013, 6(11), 5703-5716; doi:10.3390/en6115703

Measurement of Lipid Droplet Accumulation Kinetics in Chlamydomonas reinhardtii Using Seoul-Fluor

Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical Engineering, Seoul National University, Seoul 151-744, Korea
Department of Chemistry, Seoul National University, Seoul 151-744, Korea
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-744, Korea
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 31 August 2013 / Revised: 17 October 2013 / Accepted: 21 October 2013 / Published: 31 October 2013
(This article belongs to the Special Issue Algae Fuel 2013)
View Full-Text   |   Download PDF [878 KB, uploaded 17 March 2015]   |  


Alternative energy resources have become an important issue due to the limited stocks of petroleum-based fuel. Microalgae, a source of renewable biodiesel, use solar light to convert CO2 into lipid droplets (LDs). Quantification of LDs in microalgae is required for developing and optimizing algal bioprocess engineering. However, conventional quantification methods are both time and labor-intensive and difficult to apply in high-throughput screening systems. LDs in plant and mammalian cells can be visualized by staining with various fluorescence probes such as the Nile Red, BODIPY, and Seoul-Fluor (SF) series. This report describes the optimization of LD staining in Chlamydomonas reinhardtii with SF probes via systematic variations of dye concentration, staining time, temperature, and pH. A protocol for quantitative measurement of accumulation kinetics of LDs in C. reinhardtii was developed using a spectrofluorimeter and the accuracy of LD size measurement was confirmed by transmission electron microscopy (TEM). Our results indicate that our spectrofluorimeter-based measurement approach can monitor kinetics of intracellular LDs (in control and nitrogen-source-starved Chlamydomonas reinhardtii) accumulation that has not been possible in the case of conventional imaging-based methods. Our results presented here confirmed that an SF44 can be a powerful tool for in situ monitoring and tracking of intracellular LDs formation. View Full-Text
Keywords: microalgae; Chlamydomonas reinhardtii; SF44; lipid droplets; biodiesel microalgae; Chlamydomonas reinhardtii; SF44; lipid droplets; biodiesel

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Park, J.W.; Na, S.C.; Lee, Y.; Lee, S.; Park, S.B.; Jeon, N.L. Measurement of Lipid Droplet Accumulation Kinetics in Chlamydomonas reinhardtii Using Seoul-Fluor. Energies 2013, 6, 5703-5716.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Energies EISSN 1996-1073 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top