Energies 2013, 6(10), 5182-5199; doi:10.3390/en6105182
Article

Microbial Community Response to Seasonal Temperature Variation in a Small-Scale Anaerobic Digester

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Received: 18 September 2013; in revised form: 8 October 2013 / Accepted: 9 October 2013 / Published: 14 October 2013
(This article belongs to the Special Issue Biomass and Biofuels 2013)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The Bacterial and Archaeal communities in a 1.14 m3 ambient temperature anaerobic digester treating dairy cow manure were investigated using terminal restriction fragment length polymorphisms (T-RFLP) and direct sequencing of the cloned polymerase chain reaction (PCR) products. Results indicate shifts in the structure of the both the Archaeal and Bacterial communities coincided with digester re-inoculation as well as temperature and loading rate changes. Following re-inoculation of the sour digester, the predominant Archaea shifted from Methanobrevibacter to Methanosarcina, which was the most abundant Archaea in the inoculum. Methonosarcina was replaced by Methanosaeta after the resumption of digester loading in the summer of 2010. Methanosaeta began to decline in abundance as the digester temperature cooled in the fall of 2010 while Methanobrevibacter increased in abundance. The microbial community rate of change was variable during the study period, with the most rapid changes occurring after re-inoculation.
Keywords: anaerobic digestion; fixed-dome digester; microbial communities; psychrophilic; T-RFLP
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MDPI and ACS Style

Ciotola, R.J.; Martin, J.F.; Castańo, J.M.; Lee, J.; Michel, F. Microbial Community Response to Seasonal Temperature Variation in a Small-Scale Anaerobic Digester. Energies 2013, 6, 5182-5199.

AMA Style

Ciotola RJ, Martin JF, Castańo JM, Lee J, Michel F. Microbial Community Response to Seasonal Temperature Variation in a Small-Scale Anaerobic Digester. Energies. 2013; 6(10):5182-5199.

Chicago/Turabian Style

Ciotola, Richard J.; Martin, Jay F.; Castańo, Juan M.; Lee, Jiyoung; Michel, Frederick. 2013. "Microbial Community Response to Seasonal Temperature Variation in a Small-Scale Anaerobic Digester." Energies 6, no. 10: 5182-5199.

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