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Energies 2013, 6(10), 5182-5199; doi:10.3390/en6105182
Article

Microbial Community Response to Seasonal Temperature Variation in a Small-Scale Anaerobic Digester

1,* , 1
, 2
, 3,4
 and 5
1 Department of Food, Agricultural and Biological Engineering, The Ohio State University, Agricultural Engineering Building, 590 Woody Hayes Dr., Columbus, OH 43210-1057, USA 2 Facultad de Ciencias Ambientales, Universidad Tecnológica de Pereira (Faculty of Environmental Sciences, Technological University of Pereira), Apartado Aéreo 097, Pereira 660017, Colombia 3 Division of Environmental Health Sciences, College of Public Health, The Ohio State University, 406 Cunz Hall, 1841 Neil Avenue, Columbus, OH 43210-1057, USA 4 Department of Food Science and Technology, The Ohio State University, Parker Food Science and Technology Building, 2015 Fyfe Road, Columbus, OH 43210, USA 5 Department of Food, Agricultural and Biological Engineering, The Ohio State University, Agricultural Engineering Bldg. Rm. 107, OARDC, Wooster, OH 44691, USA
* Author to whom correspondence should be addressed.
Received: 18 September 2013 / Revised: 8 October 2013 / Accepted: 9 October 2013 / Published: 14 October 2013
(This article belongs to the Special Issue Biomass and Biofuels 2013)
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Abstract

The Bacterial and Archaeal communities in a 1.14 m3 ambient temperature anaerobic digester treating dairy cow manure were investigated using terminal restriction fragment length polymorphisms (T-RFLP) and direct sequencing of the cloned polymerase chain reaction (PCR) products. Results indicate shifts in the structure of the both the Archaeal and Bacterial communities coincided with digester re-inoculation as well as temperature and loading rate changes. Following re-inoculation of the sour digester, the predominant Archaea shifted from Methanobrevibacter to Methanosarcina, which was the most abundant Archaea in the inoculum. Methonosarcina was replaced by Methanosaeta after the resumption of digester loading in the summer of 2010. Methanosaeta began to decline in abundance as the digester temperature cooled in the fall of 2010 while Methanobrevibacter increased in abundance. The microbial community rate of change was variable during the study period, with the most rapid changes occurring after re-inoculation.
Keywords: anaerobic digestion; fixed-dome digester; microbial communities; psychrophilic; T-RFLP anaerobic digestion; fixed-dome digester; microbial communities; psychrophilic; T-RFLP
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ciotola, R.J.; Martin, J.F.; Castańo, J.M.; Lee, J.; Michel, F. Microbial Community Response to Seasonal Temperature Variation in a Small-Scale Anaerobic Digester. Energies 2013, 6, 5182-5199.

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