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A Novel Miniature Culture System to Screen CO2-Sequestering Microalgae
School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China
New Energy Investment Co. Ltd., Beijing 100016, China
* Author to whom correspondence should be addressed.
Received: 1 September 2012; in revised form: 23 October 2012 / Accepted: 23 October 2012 / Published: 1 November 2012
Abstract: In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO2 fixation. Cell growth under different CO2 supply conditions (0.2, 0.4, 0.8, and 1.2 g L−1 d−1), residual nitrate, and pH value of Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, and Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A sample screen of six microalgal species (Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, Selenastrum capricornutum, Scenedesmus obliquus SJTU-3, Chlamydomonas sajao, Dunaliella primolecta) was carried out in flasks and the M96SS. Chlamydomonas sajao appeared to be a robust performer (highest cell density: 1.437 g L−1) in anaerobic pond water with 0.8, and 1.2 g L−1 d−1 CO2. The reliability and efficiency of the M96SS were verified through a comparison of traditional flask culture, M96SS, Lukavský’s system, and a microplate shaker.
Keywords: microalgae; miniature; screening; CO2 fixation; wastewater
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Cite This Article
MDPI and ACS Style
Han, W.; Li, C.; Miao, X.; Yu, G. A Novel Miniature Culture System to Screen CO2-Sequestering Microalgae. Energies 2012, 5, 4372-4389.
Han W, Li C, Miao X, Yu G. A Novel Miniature Culture System to Screen CO2-Sequestering Microalgae. Energies. 2012; 5(11):4372-4389.
Han, Wei; Li, Chunying; Miao, Xiaoling; Yu, Guangxin. 2012. "A Novel Miniature Culture System to Screen CO2-Sequestering Microalgae." Energies 5, no. 11: 4372-4389.