Next Article in Journal
Mercury-Induced Externalization of Phosphatidylserine and Caspase 3 Activation in Human Liver Carcinoma (HepG2) Cells
Previous Article in Journal
Exposure to Chlorinated Biphenyls Causes Polymorphonucleocytes to Induce Progenitor Cell Toxicity in Culture
Article Menu

Export Article

Open AccessArticle
Int. J. Environ. Res. Public Health 2006, 3(1), 31-37; doi:10.3390/ijerph2006030004

Internalization of Near-Infrared Fluorescent Dyes within Isolated Leukocyte Populations

1
Department of Biology, School of Computer, Mathematical and Natural Sciences, Morgan State University, Baltimore, Maryland 21251, USA
2
Department of Chemistry , School of Computer, Mathematical and Natural Sciences, Morgan State University, Baltimore, Maryland 21251, USA
*
Author to whom correspondence should be addressed.
Received: 11 November 2005 / Accepted: 15 March 2006 / Published: 31 March 2006
View Full-Text   |   Download PDF [166 KB, uploaded 19 June 2014]   |  

Abstract

Scientists have expressed continued interest in the development of microsensor technology that can indicate toxicity that occurs within a cell after a chemical challenge. One of the more useful approaches of microsensor technology is the application of fluorescent spectroscopy to indicate early-stage injury with optimal specificity and decreased background interference. If the toxicity is detected during the early onset period of injury, then the probability for therapeutic recovery is promising. There has been increasing interest in the use of infrared (IR) and near infrared (NIR) dyes as biological microsensors due to their fluorescent spectral characteristics. Three of the most essential characteristics are the ability to minimize background interference by extraneous biological matrices, to exhibit optimal molar absorptivity and quantum yields, to maintain chronic cellular homeostasis. Therefore, the present study was developed to determine if selected NIR dyes would distribute within isolated neutrophil populations without altering normal cellular homeostasis using fluorescent wavelength analysis. The results demonstrate that selected NIR dyes undergo internalization within target cell populations while maintaining cell viability and homeostasis. In addition, these dyes demonstrate changes in absorbance and fluorescence analysis after the immune cells were challenged with a stimulant. Moreover, critical cellular functions, such as degranulation and superoxide production were not compromised by the internalization of the NIR dyes. These data suggest that selected NIR dyes can undergo intracellular uptake within neutrophil cultures without altering the normal functional capabilities of the targeted cell population.
Keywords: Scientists have expressed continued interest in the development of microsensor technology that can indicate toxicity that occurs within a cell after a chemical challenge. One of the more useful approaches of microsensor technology is the application of fluorescent spectroscopy to indicate early-stage injury with optimal specificity and decreased background interference. If the toxicity is detected during the early onset period of injury; then the probability for therapeutic recovery is promising Scientists have expressed continued interest in the development of microsensor technology that can indicate toxicity that occurs within a cell after a chemical challenge. One of the more useful approaches of microsensor technology is the application of fluorescent spectroscopy to indicate early-stage injury with optimal specificity and decreased background interference. If the toxicity is detected during the early onset period of injury; then the probability for therapeutic recovery is promising
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Baker, M.; Ntam, C.; Reese, C.T.; Martin, T.V.; Carrington, S.; Leotaub, J.; Cox, L.; Williams, R.J.; Hill, D.A. Internalization of Near-Infrared Fluorescent Dyes within Isolated Leukocyte Populations. Int. J. Environ. Res. Public Health 2006, 3, 31-37.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top