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Int. J. Environ. Res. Public Health 2006, 3(1), 23-30; doi:10.3390/ijerph2006030003
Article

Exposure to Chlorinated Biphenyls Causes Polymorphonucleocytes to Induce Progenitor Cell Toxicity in Culture

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Received: 11 November 2005; Accepted: 15 March 2006 / Published: 31 March 2006
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Abstract: Progenitor cells (PC) are the precursors for many developmental structures and are sensitive to a variety of toxic agents including the environmental contaminants, polychlorinated biphenyls (PCBs). The mechanism(s) that contributes to the development of PCB-induced progenitor cell-related fetotoxicities are not completely understood. However, several studies have demonstrated an important role for neutrophils (polymorphonucleocytes) in the development of PCB induced toxicities. Our recent findings have indicated that conditioned medium collected from PC (CMPC) exposed to a single dose of the PCB mixture, Aroclor 1248, can activate isolated neutrophil populations. Because of our recent findings, this study was conducted to determine if conditioned medium from PC treated with a PCB mixture causes neutrophils to injure PC in culture. Isolated PC were cultured and treated with different concentrations of Aroclor 1248 for 24 hours. The resulting PC-derived conditioned media was collected and its affect on neutrophil activity was analyzed. Conditioned medium from PC treated with Aroclor 1248 was chemotactic for neutrophils. The conditioned medium from Aroclor 1248 treated-PC also stimulated neutrophils to release super oxide anion, cathepsin G and elastase into culture medium. Furthermore, the conditioned medium from Aroclor 1248 treated- PC was able to stimulate neutrophils to cause progenitor cell toxicity in co-cultures. The conditioned medium from Aroclor 1248 treated-PC was not toxic to individual neutrophil cultures or PC cultures. Moreover, the addition of a protease inhibitor to the co-cultures containing neutrophils and PC, afforded protection against neutrophil-induced cytotoxicity of PC. These data suggest that a PCB mixture can cause progenitor cells to produce a factor(s) that activates neutrophils and stimulates them to damage PC populations in culture.
Keywords: Polychlorinated Biphenyls; Aroclor; Neutrophils; Polymorphonucleocytes; Progenitor cells; Toxicity Polychlorinated Biphenyls; Aroclor; Neutrophils; Polymorphonucleocytes; Progenitor cells; Toxicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hill, D.A.; Reese, C.T.; Clarke, D.; Martin, T.V. Exposure to Chlorinated Biphenyls Causes Polymorphonucleocytes to Induce Progenitor Cell Toxicity in Culture. Int. J. Environ. Res. Public Health 2006, 3, 23-30.

AMA Style

Hill DA, Reese CT, Clarke D, Martin TV. Exposure to Chlorinated Biphenyls Causes Polymorphonucleocytes to Induce Progenitor Cell Toxicity in Culture. International Journal of Environmental Research and Public Health. 2006; 3(1):23-30.

Chicago/Turabian Style

Hill, Dwayne A.; Reese, Carroll T.; Clarke, Dwane; Martin, Tanika V. 2006. "Exposure to Chlorinated Biphenyls Causes Polymorphonucleocytes to Induce Progenitor Cell Toxicity in Culture." Int. J. Environ. Res. Public Health 3, no. 1: 23-30.


Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert