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Morphological and Physiological Responses of Morning Glory (Ipomoea lacunosa L.) Grown in a Lead- and Chelate-Amended Soil
Department of Biology, Southern University at New Orleans, New Orleans, LA 70126, USA
Department of Biology, Jackson State University, Jackson, MS 39217, USA
* Author to whom correspondence should be addressed.
Received: 10 January 2005 / Accepted: 10 April 2005 / Published: 14 August 2005
Abstract: Lead (Pb) is one of the most toxic metals in the environment and may cause drastic morphological and physiological deformities in Ipomoea lacunosa. The goal of this research was to evaluate some morphological and physiological responses of morning glory grown on a Pb- and chelate-amended soil. Soil samples were analyzed, at Mississippi State University Soil Laboratory, for physico-chemical parameters, such as soil texture (73% sand, 23% silt, 4.4% clay), organic matter (6.24 ± 0.60%), and pH (7.95 ± 0.03), to establish soil conditions at the beginning of the experiments. Five EDTA (ethylenediaminetetraacetic acid) concentrations (0, 0.1, 0.5, 1, 5mM) and four lead (0, 500, 1000, 2000mg/L) treatments were arranged in factorial in a Randomized Complete Block (RCB) design with five replications. Duncan’s multiple comparison range test showed that the mean difference values of stomatal density were significant between 500 and 1000mg/L Pb and between 1000 and 2000mg/L Pb. Two way ANOVA (at 1% level) indicated that interaction between Pb and EDTA had a significant effect on the stomatal density and photosynthetic rates, and at 5% level Pb had a significant effect on chlorophyll concentrations. Lowest concentrations of chlorophyll were recorded at 2000mg/L Pb and 5mM EDTA and exhibited a decreasing trend specifically in the ranges of 1000 and 2000mg/L Pb and 1.0 and 5.0mM EDTA. Duncan’s multiple comparison range test confirmed that mean differences between the control treatment vs. 2000mg/L Pb, and 500mg/L vs. 2000mg/L Pb were significantly different at p>0.05. There was a decrease in leaf net photosynthetic rate with increasing concentrations of Pb from 0 to 2000mg/L. In conclusion, I. lacunosa L. plants were grown to maturity in all treatments with no significant and/or apparent morphological disorders, which indicated that this species might be highly tolerant even at 2000mg/L Pb concentrations in the soil.
Keywords: Lead; EDTA; phytoremediation; chlorophyll; stomata; photosynthesis; soil
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Kambhampati, M.S.; Begonia, G.B.; Begonia, M.F.T.; Bufford, Y. Morphological and Physiological Responses of Morning Glory (Ipomoea lacunosa L.) Grown in a Lead- and Chelate-Amended Soil. Int. J. Environ. Res. Public Health 2005, 2, 299-303.
Kambhampati MS, Begonia GB, Begonia MFT, Bufford Y. Morphological and Physiological Responses of Morning Glory (Ipomoea lacunosa L.) Grown in a Lead- and Chelate-Amended Soil. International Journal of Environmental Research and Public Health. 2005; 2(2):299-303.
Kambhampati, Murty S.; Begonia, Gregorio B.; Begonia, Maria F.T.; Bufford, Yolanda. 2005. "Morphological and Physiological Responses of Morning Glory (Ipomoea lacunosa L.) Grown in a Lead- and Chelate-Amended Soil." Int. J. Environ. Res. Public Health 2, no. 2: 299-303.