Next Article in Journal
Depression, Anxiety and Symptoms of Stress among Baccalaureate Nursing Students in Hong Kong: A Cross-Sectional Study
Previous Article in Journal
Newborn Parent Based Intervention to Increase Child Safety Seat Use
Article Menu

Export Article

Open AccessArticle
Int. J. Environ. Res. Public Health 2016, 13(8), 778; doi:10.3390/ijerph13080778

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

1
Biosciences Institute, São Paulo State University—UNESP, Coastal Campus, São Vicente, São Paulo 11330-900, Brazil
2
Biology Department, Edge Hill University, St. Helens Road, Lancashire, Ormskirk L39 4QP, UK
3
Richmond Pharmacology Ltd., St. George’s University London, Cranmer Terrace, London SW17 0RE, UK
4
Department of Chemical Sciences and Natural Resources, Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811-230, Chile
5
CEB-Centre of Biological Engineering, Micoteca da Universidade do Minho, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal
*
Authors to whom correspondence should be addressed.
Academic Editor: Miklas Scholz
Received: 7 June 2016 / Revised: 26 July 2016 / Accepted: 28 July 2016 / Published: 2 August 2016
View Full-Text   |   Download PDF [1400 KB, uploaded 2 August 2016]   |  

Abstract

Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L−1) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs. View Full-Text
Keywords: alkaline conditions; fixed-bed bioreactor; glycerol; Reactive Black 5; reverse transcriptase -PCR; white-rot fungus alkaline conditions; fixed-bed bioreactor; glycerol; Reactive Black 5; reverse transcriptase -PCR; white-rot fungus
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ottoni, C.; Simões, M.F.; Fernandes, S.; Santos, C.R.; Lima, N. High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs. Int. J. Environ. Res. Public Health 2016, 13, 778.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top