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Int. J. Environ. Res. Public Health 2016, 13(2), 227; doi:10.3390/ijerph13020227

Protective Effect of Onion Extract on Bleomycin-Induced Cytotoxicity and Genotoxicity in Human Lymphocytes

1
School of Public Health, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
2
Department of Biomedical and Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, University of Montana, 32 Campus Drive, Missoula, MT 59812, USA
3
Molecular Epidemiology Branch, Division of Cancer Epidemiology and Prevention, Research Institute, National Cancer Center, Ilsandong-gu, Gyeonggi-do 410-769, Korea
4
Da Vinci College of General Education, Chung-Ang University, Dongjak-Gu, Seoul 156-756, Korea
5
Institute of Health and Environment, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 31 December 2015 / Revised: 29 January 2016 / Accepted: 6 February 2016 / Published: 19 February 2016
View Full-Text   |   Download PDF [657 KB, uploaded 22 February 2016]   |  

Abstract

Following one of the world’s largest nuclear accidents, occured at Fukushima, Japan in 2011, a significant scientific effort has focused on minimizing the potential adverse health effects due to radiation exposure. The use of natural dietary antioxidants to reduce the risk of radiation-induced oxidative DNA damage is a simple strategy for minimizing radiation-related cancer rates and improving overall health. The onion is among the richest sources of dietary flavonoids and is an important food for increasing their overall intake. Therefore, we examined the effect of an onion extract on cyto- and geno-toxicity in human lymphocytes treated with bleomycin (BLM), a radiomimetic agent. In addition, we measured the frequency of micronuclei (MN) and DNA damage following treatment with BLM using a cytokinesis-blocked micronucleus assay and a single cell gel electrophoresis assay. We observed a significant increase in cell viability in lymphocytes treated with onion extract then exposed to BLM compared to cells treated with BLM alone. The frequency of BLM induced MN and DNA damage increased in a dose-dependent manner; however, when lymphocytes were pretreated with onion extract (10 and 20 μL/mL), the frequency of BLM-induced MN was decreased at all doses of BLM and DNA damage was decreased at 3 μg/mL of BLM. These results suggest that onion extract may have protective effects against BLM-induced cyto- and genotoxicity in human lymphocytes. View Full-Text
Keywords: radiation; onion extract; bleomycin; cytokinesis-blocked micronucleus assay; single cell gel electrophoresis assay radiation; onion extract; bleomycin; cytokinesis-blocked micronucleus assay; single cell gel electrophoresis assay
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MDPI and ACS Style

Cho, Y.H.; Lee, J.W.; Woo, H.D.; Lee, S.; Kim, Y.J.; Lee, Y.; Shin, S.; Joung, H.; Chung, H.W. Protective Effect of Onion Extract on Bleomycin-Induced Cytotoxicity and Genotoxicity in Human Lymphocytes. Int. J. Environ. Res. Public Health 2016, 13, 227.

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