Next Article in Journal
A Simulation Study of Categorizing Continuous Exposure Variables Measured with Error in Autism Research: Small Changes with Large Effects
Next Article in Special Issue
Detection of Antibiotic Resistant Staphylococcus aureus from Milk: A Public Health Implication
Previous Article in Journal
Identification of Major Risk Sources for Surface Water Pollution by Risk Indexes (RI) in the Multi-Provincial Boundary Region of the Taihu Basin, China
Previous Article in Special Issue
Microbiological Food Safety for Vulnerable People
Article Menu

Export Article

Open AccessArticle
Int. J. Environ. Res. Public Health 2015, 12(8), 10171-10197; doi:10.3390/ijerph120810171

Characterization of the Bacterial Community Naturally Present on Commercially Grown Basil Leaves: Evaluation of Sample Preparation Prior to Culture-Independent Techniques

1
Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation (LFMFP), Ghent University, Ghent 9000, Belgium
2
Faculty of Pharmaceutical Sciences, Department of Pharmaceutics, Laboratory of Pharmaceutical Biotechnology (LabFBT), Ghent University, Ghent 9000, Belgium
3
Faculty of Bioscience Engineering, Department of Biochemical and Microbial Technology, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Ghent 9000, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 7 July 2015 / Revised: 18 August 2015 / Accepted: 19 August 2015 / Published: 21 August 2015
(This article belongs to the Special Issue Food Safety)
View Full-Text   |   Download PDF [2562 KB, uploaded 24 August 2015]   |  

Abstract

Fresh herbs such as basil constitute an important food commodity worldwide. Basil provides considerable culinary and health benefits, but has also been implicated in foodborne illnesses. The naturally occurring bacterial community on basil leaves is currently unknown, so the epiphytic bacterial community was investigated using the culture-independent techniques denaturing gradient gel electrophoresis (DGGE) and next-generation sequencing (NGS). Sample preparation had a major influence on the results from DGGE and NGS: Novosphingobium was the dominant genus for three different basil batches obtained by maceration of basil leaves, while washing of the leaves yielded lower numbers but more variable dominant bacterial genera including Klebsiella, Pantoea, Flavobacterium, Sphingobacterium and Pseudomonas. During storage of basil, bacterial growth and shifts in the bacterial community were observed with DGGE and NGS. Spoilage was not associated with specific bacterial groups and presumably caused by physiological tissue deterioration and visual defects, rather than by bacterial growth. View Full-Text
Keywords: Novosphingobium; fresh herbs; 16S rRNA; DGGE; next-generation sequencing NGS Novosphingobium; fresh herbs; 16S rRNA; DGGE; next-generation sequencing NGS
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ceuppens, S.; Delbeke, S.; De Coninck, D.; Boussemaere, J.; Boon, N.; Uyttendaele, M. Characterization of the Bacterial Community Naturally Present on Commercially Grown Basil Leaves: Evaluation of Sample Preparation Prior to Culture-Independent Techniques. Int. J. Environ. Res. Public Health 2015, 12, 10171-10197.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top