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Int. J. Environ. Res. Public Health 2015, 12(8), 10079-10092; doi:10.3390/ijerph120810079

Prevalence of Genes of OXA-23 Carbapenemase and AdeABC Efflux Pump Associated with Multidrug Resistance of Acinetobacter baumannii Isolates in the ICU of a Comprehensive Hospital of Northwestern China

1,2
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1,3
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2,4
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1,2
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1
and
1,2,*
1
Ningxia Key laboratory of Clinical and Pathogenic Microbiology, Yinchuan, Ningxia 750004, China
2
Center of Laboratory Medicine, the General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China
3
The Graduate school, Ningxia Medical University, Yinchuan, Ningxia 750004, China
4
The First People's Hospital of Mudanjiang City, Mudanjiang, Heilongjiang 157011, China
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 11 July 2015 / Revised: 10 August 2015 / Accepted: 17 August 2015 / Published: 21 August 2015
View Full-Text   |   Download PDF [326 KB, uploaded 21 August 2015]   |  

Abstract

The objective of this study was to explore the molecular epidemiology and the genetic support of clinical multidrug resistant (MDR) Acinetobacter baumannii (A. baumannii) isolates in an ICU ward of a comprehensive hospital. A total of 102 non-duplicate drug-resistant A. baumannii isolates were identified and 93 (91.1%) of them were MDR strains. Molecular analysis demonstrated that carbapenemase genes blaOXA-23 and blaOXA-51 were presented in all 93 MDR isolates (100%), but other carbapenemase genes, including blaOXA-24, blaOXA-58, blaIMP-1, blaIMP-4, blaSIM, and blaVIM genes were completely absent in all isolates. In addition, genes of AdeABC efflux system were detected in 88.2% (90/102) isolates. Interestingly, an addition to efflux pump inhibitor, reserpine could significantly enhance the susceptibility of MDR isolates to moxifloxacin, cefotaxime, and imipenem (p < 0.01). Clonal relationship analysis further grouped these clinical drug-resistant isolates into nine clusters, and the MDR strains were mainly in clusters A, B, C, and D, which include 16, 13, 25, and 15 isolates, respectively. This study demonstrated that clinical isolates carrying carbapenemase-encoding genes blaOXA-23 and AdeABC efflux pump genes are the main prevalent MDR A. baumannii, and the co-expression of oxacillinase and efflux pump proteins are thus considered to be the important reason for the prevalence of this organism in the ICU of this hospital. View Full-Text
Keywords: Acinetobacter baumannii; multidrug resistance; nosocomial infections; oxacillinase; efflux pump Acinetobacter baumannii; multidrug resistance; nosocomial infections; oxacillinase; efflux pump
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Jia, W.; Li, C.; Zhang, H.; Li, G.; Liu, X.; Wei, J. Prevalence of Genes of OXA-23 Carbapenemase and AdeABC Efflux Pump Associated with Multidrug Resistance of Acinetobacter baumannii Isolates in the ICU of a Comprehensive Hospital of Northwestern China. Int. J. Environ. Res. Public Health 2015, 12, 10079-10092.

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