Next Article in Journal
Retrospective Investigation of a Lead Poisoning Outbreak from the Consumption of an Ayurvedic Medicine: Durban, South Africa
Next Article in Special Issue
Effect of Disinfectants on Preventing the Cross-Contamination of Pathogens in Fresh Produce Washing Water
Previous Article in Journal
Short-Term Effects of Fine Particulate Matter and Temperature on Lung Function among Healthy College Students in Wuhan, China
Previous Article in Special Issue
Irrigation Water Quality for Leafy Crops: A Perspective of Risks and Potential Solutions
Article Menu

Export Article

Open AccessCommunication
Int. J. Environ. Res. Public Health 2015, 12(7), 7794-7803; doi:10.3390/ijerph120707794

Effect of Neem (Azadirachta indica) on the Survival of Escherichia coli O157:H7 in Dairy Manure

1
Produce Safety and Microbiology Research Unit, United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, USA
2
Foodborne Toxin Detection and Prevention Research Unit, United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Mieke Uyttendaele, Eelco Franz and Oliver Schlüter
Received: 6 March 2015 / Revised: 1 July 2015 / Accepted: 6 July 2015 / Published: 10 July 2015
(This article belongs to the Special Issue Food Safety)
View Full-Text   |   Download PDF [738 KB, uploaded 10 July 2015]   |  

Abstract

Escherichia coli O157:H7 (EcO157) shed in cattle manure can survive for extended periods of time and intervention strategies to control this pathogen at the source are critical as produce crops are often grown in proximity to animal raising operations. This study evaluated whether neem (Azadirachta indica), known for its antimicrobial and insecticidal properties, can be used to amend manure to control EcO157. The influence of neem materials (leaf, bark, and oil) on the survival of an apple juice outbreak strain of EcO157 in dairy manure was monitored. Neem leaf and bark supplements eliminated the pathogen in less than 10 d with a D-value (days for 90% elimination) of 1.3 d. In contrast, nearly 4 log CFU EcO157/g remained after 10 d in neem-free manure control. The ethyl acetate extractable fraction of neem leaves was inhibitory to the growth of EcO157 in LB broth. Azadirachtin, a neem product with insect antifeedant properties, failed to inhibit EcO157. Application of inexpensive neem supplements to control pathogens in manure and possibly in produce fields may be an option for controlling the transfer of foodborne pathogens from farm to fork. View Full-Text
Keywords: neem; Azadirachta indica; E. coli O157:H7; bioscreen; survival; dairy manure; neem extracts; azadirachtin neem; Azadirachta indica; E. coli O157:H7; bioscreen; survival; dairy manure; neem extracts; azadirachtin
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ravva, S.V.; Korn, A. Effect of Neem (Azadirachta indica) on the Survival of Escherichia coli O157:H7 in Dairy Manure. Int. J. Environ. Res. Public Health 2015, 12, 7794-7803.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top