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Int. J. Environ. Res. Public Health 2015, 12(10), 13069-13084; doi:10.3390/ijerph121013069

Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF

1
College of Environment and Plant Protection, Hainan University, Haikou 570228, China
2
School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China
3
Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou 570228, China
*
Author to whom correspondence should be addressed.
Academic Editors: Huixiao Hong and Paul B. Tchounwou
Received: 4 August 2015 / Revised: 2 October 2015 / Accepted: 9 October 2015 / Published: 16 October 2015
(This article belongs to the Special Issue Endocrine Disruptors and Public Health)
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Abstract

Bisphenol AF (BPAF) is extensively used as a raw material in industry, resulting in its widespread distribution in the aqueous environment. However, the effect of BPAF on the hypothalamic-pituitary-thyroidal (HPT) axis remains unknown. For elucidating the disruptive effects of BPAF on thyroid function and expression of the representative genes along the HPT axis in zebrafish (Danio rerio) embryos, whole-body total 3,3′,5-triiodothyronine (TT3), total 3,5,3′,5′-tetraiodothyronine (TT4), free 3,3′,5-triiodothyronine (FT3) and free 3,5,3′,5′-tetraiodothyronine (FT4) levels were examined following 168 h post-fertilization exposure to different BPAF concentrations (0, 5, 50 and 500 μg/L). The results showed that whole-body TT3, TT4, FT3 and FT4 contents decreased significantly with the BPAF treatment, indicating an endocrine disruption of thyroid. The expression of thyroid-stimulating hormone-β and thyroglobulin genes increased after exposing to 50 μg/L BPAF in seven-day-old larvae. The expressions of thyronine deiodinases type 1, type 2 and transthyretin mRNAs were also significantly up-regulated, which were possibly associated with a deterioration of thyroid function. However, slc5a5 gene transcription was significantly down-regulated at 50 μg/L and 500 μg/L BPAF exposure. Furthermore, trα and trβ genes were down-regulated transcriptionally after BPAF exposure. It demonstrates that BPAF exposure triggered thyroid endocrine toxicity by altering the whole-body contents of thyroid hormones and changing the transcription of the genes involved in the HPT axis in zebrafish larvae. View Full-Text
Keywords: bisphenol AF (BPAF); zebrafish (Danio rerio); thyroid hormones (THs); hypothalamic-pituitary-thyroid (HPT) axis bisphenol AF (BPAF); zebrafish (Danio rerio); thyroid hormones (THs); hypothalamic-pituitary-thyroid (HPT) axis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tang, T.; Yang, Y.; Chen, Y.; Tang, W.; Wang, F.; Diao, X. Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF. Int. J. Environ. Res. Public Health 2015, 12, 13069-13084.

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