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Int. J. Environ. Res. Public Health 2015, 12(10), 12002-12015; doi:10.3390/ijerph121012002

Bacterial Community Diversity of Oil-Contaminated Soils Assessed by High Throughput Sequencing of 16S rRNA Genes

College of Life Science, Northeast Forestry University, No.26 Hexing Street, Xiangfang District, Harbin 150040, China
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Author to whom correspondence should be addressed.
Academic Editor: Yu-Pin Lin
Received: 27 July 2015 / Revised: 27 August 2015 / Accepted: 18 September 2015 / Published: 24 September 2015
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Abstract

Soil bacteria play a major role in ecological and biodegradable function processes in oil-contaminated soils. Here, we assessed the bacterial diversity and changes therein in oil-contaminated soils exposed to different periods of oil pollution using 454 pyrosequencing of 16S rRNA genes. No less than 24,953 valid reads and 6246 operational taxonomic units (OTUs) were obtained from all five studied samples. OTU richness was relatively higher in contaminated soils than clean samples. Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Planctomycetes and Proteobacteria were the dominant phyla among all the soil samples. The heatmap plot depicted the relative percentage of each bacterial family within each sample and clustered five samples into two groups. For the samples, bacteria in the soils varied at different periods of oil exposure. The oil pollution exerted strong selective pressure to propagate many potentially petroleum degrading bacteria. Redundancy analysis (RDA) indicated that organic matter was the highest determinant factor for explaining the variations in community compositions. This suggests that compared to clean soils, oil-polluted soils support more diverse bacterial communities and soil bacterial community shifts were mainly controlled by organic matter and exposure time. These results provide some useful information for bioremediation of petroleum contaminated soil in the future. View Full-Text
Keywords: oil-contaminated soils; 454 Pyrosequencing; 16S rRNA; bacterial community; bacterial diversity oil-contaminated soils; 454 Pyrosequencing; 16S rRNA; bacterial community; bacterial diversity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Peng, M.; Zi, X.; Wang, Q. Bacterial Community Diversity of Oil-Contaminated Soils Assessed by High Throughput Sequencing of 16S rRNA Genes. Int. J. Environ. Res. Public Health 2015, 12, 12002-12015.

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