Abstract: A total of 110 strains of Klebsiella pneumoniae were used to optimize pulsed-field gel electrophoresis (PFGE) for subtyping of K. pneumoniae. For optimization of electrophoresis parameters (EPs) of XbaI-PFGE, 11 isolates were analyzed with XbaI digestion using three EPs. The EP of a switch time of 6 to 36 s for 18.5 h gave clearest patterns and was declared the optimal EP for XbaI PFGE of K. pneumoniae. By software analysis and pilot study, AvrII was chosen as another PFGE enzyme. Both XbaI- and AvrII-PFGE gave D-values higher than 0.99 for 69 K. pneumoniae isolated from different sources. Our results also showed good typeability, reproducibility of both XbaI- and AvrII-PFGE for K. pneumoniae subtyping. Furthermore, the established PFGE method also had good discriminatory power to distinguish outbreak K. pneumoniae strains and a high degree of consistency with multilocus sequence typing method. A rapid PFGE protocol was established here, which could be used for genotyping and other researches of K. pneumoniae.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Han, H.; Zhou, H.; Li, H.; Gao, Y.; Lu, Z.; Hu, K.; Xu, B. Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae. Int. J. Environ. Res. Public Health 2013, 10, 2720-2731.
Han H, Zhou H, Li H, Gao Y, Lu Z, Hu K, Xu B. Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae. International Journal of Environmental Research and Public Health. 2013; 10(7):2720-2731.
Han, Hui; Zhou, Haijian; Li, Haishan; Gao, Yuan; Lu, Zhi; Hu, Kongxin; Xu, Baoliang. 2013. "Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae." Int. J. Environ. Res. Public Health 10, no. 7: 2720-2731.