Effect of the Environmental Pollutant Hexachlorobenzene (HCB) on the Neuronal Differentiation of Mouse Embryonic Stem Cells †
Abstract
:1. Introduction
2. Experimental Section
2.1. ES Cell Culture
2.2. GABAergic and Glutamatergic Neuronal Differentiation
2.3. HCB Treatment and Cell Viability Determination
2.4. Immunofluorescence Analyses
2.5. RT-PCR Analyses
2.6. ROS Assay
2.7. NAC Treatment
2.8. Neurite Lenght Measurements
2.9. Electrophysiology Recordings
3. Results
3.1. Dose-Response Effect of HCB on Cell Viability
3.2. Expression of Lineage Markers in Encapsulated ES Cells by RT-PCR
3.3. Low Dose HCB Affects GABAergic Neuronal Maturation
3.4. HCB Induces ROS Production in GABAergic Cells
3.5. Inhibition of ROS Production Restores Neurite Outgrowth in HCB-Treated Neurons
3.6. Electrophysiology Analyses
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Addae, C.; Cheng, H.; Martinez-Ceballos, E. Effect of the Environmental Pollutant Hexachlorobenzene (HCB) on the Neuronal Differentiation of Mouse Embryonic Stem Cells. Int. J. Environ. Res. Public Health 2013, 10, 5244-5256. https://doi.org/10.3390/ijerph10105244
Addae C, Cheng H, Martinez-Ceballos E. Effect of the Environmental Pollutant Hexachlorobenzene (HCB) on the Neuronal Differentiation of Mouse Embryonic Stem Cells. International Journal of Environmental Research and Public Health. 2013; 10(10):5244-5256. https://doi.org/10.3390/ijerph10105244
Chicago/Turabian StyleAddae, Cynthia, Henrique Cheng, and Eduardo Martinez-Ceballos. 2013. "Effect of the Environmental Pollutant Hexachlorobenzene (HCB) on the Neuronal Differentiation of Mouse Embryonic Stem Cells" International Journal of Environmental Research and Public Health 10, no. 10: 5244-5256. https://doi.org/10.3390/ijerph10105244