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Mar. Drugs 2011, 9(7), 1293-1306; doi:10.3390/md9071293

In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera

1
Unit of Biotoxines, Pasteur Institut of Tunis, 13, Place Pasteur, B.P. 74. 1002 Tunis-Belvedere, Tunisia
2
Laboratory of Immunopathology, Vaccinology and Molecular Genetics (LIVGM), WHO Collaborating Center for Research and Training in Leishmaniasis and International Associated Laboratory (LIA-CNRS), Pasteur Institut of Tunis, 13, Place Pasteur, B.P. 74. 1002 Tunis Belvedere, Tunisia
3
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece
These authors contributed equally to this work.
This author deceased on 12 May 2010.
§
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 11 May 2011 / Revised: 3 June 2011 / Accepted: 5 July 2011 / Published: 22 July 2011
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Abstract

Neorogioltriol is a tricyclic brominated diterpenoid isolated from the organic extract of the red algae Laurencia glandulifera. In the present study, the anti-inflammatory effects of neorogioltriol were evaluated both in vivo using carrageenan-induced paw edema and in vitro on lipopolysaccharide (LPS)-treated Raw264.7 macrophages. The in vivo study demonstrated that the administration of 1 mg/kg of neorogioltriol resulted in the significant reduction of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-κB-dependent luciferase reporter. This effect on NF-κB activation is not mediated through MAPK pathways. The inhibition of NF-κB activity correlates with decreased levels of LPS-induced tumor necrosis factor-alpha (TNFα) present in neorogioltriol treated supernatant cell culture. Further analyses indicated that this product also significantly inhibited the release of nitric oxide and the expression of cyclooxygenase-2 (COX-2) in LPS-stimulated Raw264.7 cells. These latter effects could only be observed for neorogioltriol concentrations below 62.5 µM. To our knowledge, this is the first report describing a molecule derived from Laurencia glandulifera with anti-inflammatory activity both in vivo and in vitro. The effect demonstrated in vitro may be explained by the inhibition of the LPS-induced NF-κB activation and TNFα production. NO release and COX-2 expression may reinforce this effect. View Full-Text
Keywords: anti-inflammatory; neorogioltriol; Laurencia; TNFα; NF-κB; NO; COX-2; carrageenan anti-inflammatory; neorogioltriol; Laurencia; TNFα; NF-κB; NO; COX-2; carrageenan
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chatter, R.; Othman, R.B.; Rabhi, S.; Kladi, M.; Tarhouni, S.; Vagias, C.; Roussis, V.; Guizani-Tabbane, L.; Kharrat, R. In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera. Mar. Drugs 2011, 9, 1293-1306.

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