Mar. Drugs 2011, 9(7), 1293-1306; doi:10.3390/md9071293
Article

In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera

Rim Chatter 1,† email, Rym Ben Othman 2,† email, Sameh Rabhi 2 email, Maria Kladi 3 email, Safa Tarhouni 1 email, Constantinos Vagias 3,‡ email, Vassilios Roussis 3 email, Lamia Guizani-Tabbane 2,§,* email and Riadh Kharrat 1,§ email
1 Unit of Biotoxines, Pasteur Institut of Tunis, 13, Place Pasteur, B.P. 74. 1002 Tunis-Belvedere, Tunisia 2 Laboratory of Immunopathology, Vaccinology and Molecular Genetics (LIVGM), WHO Collaborating Center for Research and Training in Leishmaniasis and International Associated Laboratory (LIA-CNRS), Pasteur Institut of Tunis, 13, Place Pasteur, B.P. 74. 1002 Tunis Belvedere, Tunisia 3 Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece † These authors contributed equally to this work. ‡ This author deceased on 12 May 2010. § These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 11 May 2011; in revised form: 3 June 2011 / Accepted: 5 July 2011 / Published: 22 July 2011
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Abstract: Neorogioltriol is a tricyclic brominated diterpenoid isolated from the organic extract of the red algae Laurencia glandulifera. In the present study, the anti-inflammatory effects of neorogioltriol were evaluated both in vivo using carrageenan-induced paw edema and in vitro on lipopolysaccharide (LPS)-treated Raw264.7 macrophages. The in vivo study demonstrated that the administration of 1 mg/kg of neorogioltriol resulted in the significant reduction of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-κB-dependent luciferase reporter. This effect on NF-κB activation is not mediated through MAPK pathways. The inhibition of NF-κB activity correlates with decreased levels of LPS-induced tumor necrosis factor-alpha (TNFα) present in neorogioltriol treated supernatant cell culture. Further analyses indicated that this product also significantly inhibited the release of nitric oxide and the expression of cyclooxygenase-2 (COX-2) in LPS-stimulated Raw264.7 cells. These latter effects could only be observed for neorogioltriol concentrations below 62.5 µM. To our knowledge, this is the first report describing a molecule derived from Laurencia glandulifera with anti-inflammatory activity both in vivo and in vitro. The effect demonstrated in vitro may be explained by the inhibition of the LPS-induced NF-κB activation and TNFα production. NO release and COX-2 expression may reinforce this effect.
Keywords: anti-inflammatory; neorogioltriol; Laurencia; TNFα; NF-κB; NO; COX-2; carrageenan

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MDPI and ACS Style

Chatter, R.; Othman, R.B.; Rabhi, S.; Kladi, M.; Tarhouni, S.; Vagias, C.; Roussis, V.; Guizani-Tabbane, L.; Kharrat, R. In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera. Mar. Drugs 2011, 9, 1293-1306.

AMA Style

Chatter R, Othman RB, Rabhi S, Kladi M, Tarhouni S, Vagias C, Roussis V, Guizani-Tabbane L, Kharrat R. In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera. Marine Drugs. 2011; 9(7):1293-1306.

Chicago/Turabian Style

Chatter, Rim; Othman, Rym Ben; Rabhi, Sameh; Kladi, Maria; Tarhouni, Safa; Vagias, Constantinos; Roussis, Vassilios; Guizani-Tabbane, Lamia; Kharrat, Riadh. 2011. "In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera." Mar. Drugs 9, no. 7: 1293-1306.

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