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Proteomic Analysis of Anti-Tumor Effects of 11-Dehydrosinulariolide on CAL-27 Cells
Department of Nursing, Meiho Universtity, Pingtung 91202, Taiwan
Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan
Department of Food Science and Nutrition, Meiho Universtity, Pingtung 91202, Taiwan
National Museum of Marine Biology and Aquarium, Checheng, Pingtung 94446, Taiwan
Department of Biological Science and Technology, Meiho Universtity, Pingtung 91202, Taiwan
Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 80761, Taiwan
These authors contributed equally to this work
* Author to whom correspondence should be addressed.
Received: 27 May 2011; in revised form: 27 June 2011 / Accepted: 7 July 2011 / Published: 14 July 2011
Abstract: The anti-tumor effects of 11-dehydrosinulariolide, an active ingredient isolated from soft coral Sinularia leptoclados, on CAL-27 cells were investigated in this study. In the MTT assay for cell proliferation, increasing concentrations of 11-dehydrosinulariolide decreased CAL-27 cell viability. When a concentration of 1.5 µg/mL of 11-dehydrosinulariolide was applied, the CAL-27 cells viability was reduced to a level of 70% of the control sample. The wound healing function decreased as the concentration of 11-dehydrosinulariolide increased. The results in this study indicated that treatment with 11-dehydrosinulariolide for 6 h significantly induced both early and late apoptosis of CAL-27 cells, observed by flow cytometric measurement and microscopic fluorescent observation. A comparative proteomic analysis was conducted to investigate the effects of 11-dehydrosinulariolide on CAL-27 cells at the molecular level by comparison between the protein profiling (revealed on a 2-DE map) of CAL-27 cells treated with 11-dehydrosinulariolide and that of CAL-27 cells without the treatment. A total of 28 differential proteins (12 up-regulated and 16 down-regulated) in CAL-27 cells treated with 11-dehydrosinulariolide have been identified by LC-MS/MS analysis. Some of the differential proteins are associated with cell proliferation, apoptosis, protein synthesis, protein folding, and energy metabolism. The results of this study provided clues for the investigation of biochemical mechanisms of the anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells and could be valuable information for drug development and progression monitoring of oral squamous cell carcinoma (OSCC).
Keywords: 11-dehydrosinulariolide; CAL-27 cells; proteomic analysis
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Liu, C.-I.; Chen, C.-C.; Chen, J.-C.; Su, J.-H.; Huang, H.H.; Chen, J.-F.; Wu, Y.-J. Proteomic Analysis of Anti-Tumor Effects of 11-Dehydrosinulariolide on CAL-27 Cells. Mar. Drugs 2011, 9, 1254-1272.
Liu C-I, Chen C-C, Chen J-C, Su J-H, Huang HH, Chen J-F, Wu Y-J. Proteomic Analysis of Anti-Tumor Effects of 11-Dehydrosinulariolide on CAL-27 Cells. Marine Drugs. 2011; 9(7):1254-1272.
Liu, Chih-I; Chen, Cheng-Chi; Chen, Jiing-Chuan; Su, Jui-Hsin; Huang, Han Hsiang; Chen, Jeff Yi-Fu; Wu, Yu-Jen. 2011. "Proteomic Analysis of Anti-Tumor Effects of 11-Dehydrosinulariolide on CAL-27 Cells." Mar. Drugs 9, no. 7: 1254-1272.