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Mar. Drugs 2011, 9(7), 1232-1242; doi:10.3390/md9071232
Article

Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay

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,
 and
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Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
* Author to whom correspondence should be addressed.
Received: 23 May 2011 / Revised: 28 June 2011 / Accepted: 5 July 2011 / Published: 11 July 2011
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Abstract

The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72% flocculating activity) and ammonium chloride (91% flocculating activity) were used as sole sources of carbon and nitrogen, respectively; an initial pH 6.2 of the production medium; and Mg2+ as cation. Chemical analysis of the purified bioflocculant revealed the compound to be a polysaccharide.
Keywords: marine; Algoa bay; Bacillus sp. Gilbert; bioflocculant; polysaccharide marine; Algoa bay; Bacillus sp. Gilbert; bioflocculant; polysaccharide
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Nontembiso, P.; Sekelwa, C.; Leonard, M.V.; Anthony, O.I. Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay. Mar. Drugs 2011, 9, 1232-1242.

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