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Mar. Drugs 2011, 9(7), 1232-1242; doi:10.3390/md9071232

Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay

Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
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Received: 23 May 2011 / Revised: 28 June 2011 / Accepted: 5 July 2011 / Published: 11 July 2011
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Abstract

The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72% flocculating activity) and ammonium chloride (91% flocculating activity) were used as sole sources of carbon and nitrogen, respectively; an initial pH 6.2 of the production medium; and Mg2+ as cation. Chemical analysis of the purified bioflocculant revealed the compound to be a polysaccharide.
Keywords: marine; Algoa bay; Bacillus sp. Gilbert; bioflocculant; polysaccharide marine; Algoa bay; Bacillus sp. Gilbert; bioflocculant; polysaccharide
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Nontembiso, P.; Sekelwa, C.; Leonard, M.V.; Anthony, O.I. Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay. Mar. Drugs 2011, 9, 1232-1242.

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