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Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells
Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil
Federal Institute of Education, Science and Technology of Rio Grande do Norte (IFRN), Santa Cruz-RN, Brazil
* Author to whom correspondence should be addressed.
Received: 22 February 2011; in revised form: 29 March 2011 / Accepted: 8 April 2011 / Published: 14 April 2011
Abstract: Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated L-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NFκB were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer.
Keywords: fucoidan; sulfated polysaccharides; anticancer; apoptosis-inducing factor (AIF)
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Silva Costa, L.; Silva Telles, C.B.; Medeiros Oliveira, R.; Duarte Barreto Nobre, L.T.; Dantas-Santos, N.; Barros Gomes Camara, R.; Santana Santos Pereira Costa, M.; Almeida-Lima, J.; Melo-Silveira, R.F.; Lopes Albuquerque, I.R.; Leite, E.L.; Oliveira Rocha, H.A. Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells. Mar. Drugs 2011, 9, 603-614.
Silva Costa L, Silva Telles CB, Medeiros Oliveira R, Duarte Barreto Nobre LT, Dantas-Santos N, Barros Gomes Camara R, Santana Santos Pereira Costa M, Almeida-Lima J, Melo-Silveira RF, Lopes Albuquerque IR, Leite EL, Oliveira Rocha HA. Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells. Marine Drugs. 2011; 9(4):603-614.
Silva Costa, Leandro; Silva Telles, Cinthia Beatrice; Medeiros Oliveira, Ruth; Duarte Barreto Nobre, Leonardo Thiago; Dantas-Santos, Nednaldo; Barros Gomes Camara, Rafael; Santana Santos Pereira Costa, Mariana; Almeida-Lima, Jailma; Melo-Silveira, Raniere Fagundes; Lopes Albuquerque, Ivan Rui; Leite, Edda Lisboa; Oliveira Rocha, Hugo Alexandre. 2011. "Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells." Mar. Drugs 9, no. 4: 603-614.