Mar. Drugs 2011, 9(4), 603-614; doi:10.3390/md9040603

Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells

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Received: 22 February 2011; in revised form: 29 March 2011 / Accepted: 8 April 2011 / Published: 14 April 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated L-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NFκB were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer.
Keywords: fucoidan; sulfated polysaccharides; anticancer; apoptosis-inducing factor (AIF)
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MDPI and ACS Style

Silva Costa, L.; Silva Telles, C.B.; Medeiros Oliveira, R.; Duarte Barreto Nobre, L.T.; Dantas-Santos, N.; Barros Gomes Camara, R.; Santana Santos Pereira Costa, M.; Almeida-Lima, J.; Melo-Silveira, R.F.; Lopes Albuquerque, I.R.; Leite, E.L.; Oliveira Rocha, H.A. Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells. Mar. Drugs 2011, 9, 603-614.

AMA Style

Silva Costa L, Silva Telles CB, Medeiros Oliveira R, Duarte Barreto Nobre LT, Dantas-Santos N, Barros Gomes Camara R, Santana Santos Pereira Costa M, Almeida-Lima J, Melo-Silveira RF, Lopes Albuquerque IR, Leite EL, Oliveira Rocha HA. Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells. Marine Drugs. 2011; 9(4):603-614.

Chicago/Turabian Style

Silva Costa, Leandro; Silva Telles, Cinthia Beatrice; Medeiros Oliveira, Ruth; Duarte Barreto Nobre, Leonardo Thiago; Dantas-Santos, Nednaldo; Barros Gomes Camara, Rafael; Santana Santos Pereira Costa, Mariana; Almeida-Lima, Jailma; Melo-Silveira, Raniere Fagundes; Lopes Albuquerque, Ivan Rui; Leite, Edda Lisboa; Oliveira Rocha, Hugo Alexandre. 2011. "Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells." Mar. Drugs 9, no. 4: 603-614.

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