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Mar. Drugs 2011, 9(4), 603-614; doi:10.3390/md9040603
Article

Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells

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1 Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil 2 Federal Institute of Education, Science and Technology of Rio Grande do Norte (IFRN), Santa Cruz-RN, Brazil
* Author to whom correspondence should be addressed.
Received: 22 February 2011 / Revised: 29 March 2011 / Accepted: 8 April 2011 / Published: 14 April 2011
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Abstract

Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated L-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NFκB were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer.
Keywords: fucoidan; sulfated polysaccharides; anticancer; apoptosis-inducing factor (AIF) fucoidan; sulfated polysaccharides; anticancer; apoptosis-inducing factor (AIF)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Silva Costa, L.; Silva Telles, C.B.; Medeiros Oliveira, R.; Duarte Barreto Nobre, L.T.; Dantas-Santos, N.; Barros Gomes Camara, R.; Santana Santos Pereira Costa, M.; Almeida-Lima, J.; Melo-Silveira, R.F.; Lopes Albuquerque, I.R.; Leite, E.L.; Oliveira Rocha, H.A. Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells. Mar. Drugs 2011, 9, 603-614.

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