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Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage
LAFFEX—Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí-CMRV, 64202-020, Parnaíba, PI, Brazil
LAFICA—Laboratory of Pharmacology of Inflammation and Cancer, Department of Physiology and Pharmacology, Federal University of Ceará, 60430-270, Fortaleza, CE, Brazil
Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60455-760, Fortaleza, CE, Brazil
* Author to whom correspondence should be addressed.
Received: 14 September 2011; in revised form: 17 October 2011 / Accepted: 24 October 2011 / Published: 2 November 2011
Abstract: The aim of the present study was to investigate the gastroprotective activity of a sulfated-polysaccharide (PLS) fraction extracted from the marine red algae Gracilaria caudata and the mechanism underlying the gastroprotective activity. Male Swiss mice were treated with PLS (3, 10, 30 and 90 mg·kg−1, p.o.), and after 30 min, they were administered 50% ethanol (0.5 mL/25 g−1, p.o.). One hour later, gastric damage was measured using a planimeter. Samples of the stomach tissue were also obtained for histopathological assessment and for assays of glutathione (GSH) and malondialdehyde (MDA). Other groups were pretreated with l-NAME (10 mg·kg−1, i.p.), dl-propargylglycine (PAG, 50 mg·kg−1, p.o.) or glibenclamide (5 mg·kg−1, i.p.). After 1 h, PLS (30 mg·kg−1, p.o.) was administered. After 30 min, ethanol 50% was administered (0.5 mL/25g−1, p.o.), followed by sacrifice after 60 min. PLS prevented-ethanol-induced macroscopic and microscopic gastric injury in a dose-dependent manner. However, treatment with l-NAME or glibenclamide reversed this gastroprotective effect. Administration of propargylglycine did not influence the effect of PLS. Our results suggest that PLS has a protective effect against ethanol-induced gastric damage in mice via activation of the NO/KATP pathway.
Keywords: polysaccharide; gastric damage; ethanol; nitric oxide; hydrogen sulfide
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Silva, R.O.; Santos, G.M.P.; Nicolau, L.A.D.; Lucetti, L.T.; Santana, A.P.M.; Chaves, L.S.; Barros, F.C.N.; Freitas, A.L.P.; Souza, M.H.L.P.; Medeiros, J.-V. Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage. Mar. Drugs 2011, 9, 2188-2200.
Silva RO, Santos GMP, Nicolau LAD, Lucetti LT, Santana APM, Chaves LS, Barros FCN, Freitas ALP, Souza MHLP, Medeiros J-V. Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage. Marine Drugs. 2011; 9(11):2188-2200.
Silva, Renan Oliveira; Santos, Geice Maria Pereira dos; Nicolau, Lucas Antonio Duarte; Lucetti, Larisse Tavares; Santana, Ana Paula Macedo; Chaves, Luciano de Souza; Barros, Francisco Clark Nogueira; Freitas, Ana Lúcia Ponte; Souza, Marcellus Henrique Loiola Ponte; Medeiros, Jand-Venes Rolim. 2011. "Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage." Mar. Drugs 9, no. 11: 2188-2200.