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• Jin, W
• Wang, J
• Ren, S
• Song, N
• Zhang, Q
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• Wang, J
• Ren, S
• Song, N
• Zhang, Q
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• Wang, J
• Ren, S
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• Zhang, Q

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Mar. Drugs 2012, 10(10), 2138-2152; doi:10.3390/md10102138

Article

Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)

Weihua Jin ^1,2 , Jing Wang ^1,3 , Sumei Ren ⁴ , Ni Song ⁴  and Quanbin Zhang ^1,*

¹ Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China ² University of Chinese Academy of Sciences, Beijing 100049, China ³ Nantong Marine Science and Technology R & D Center, IOCAS, Jiangsu 226006, China ⁴ College of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, China

* Author to whom correspondence should be addressed.

Received: 17 August 2012; in revised form: 10 September 2012 / Accepted: 13 September 2012 / Published: 25 September 2012

(This article belongs to the Special Issue Marine Polysaccharides)

Download PDF Full-Text [1964 KB, uploaded 25 September 2012 14:40 CEST]

Abstract: A fucoidan extracted from Saccharina japonica was fractionated by anion exchange chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and gel filtration chromatography while Fraction Y2 was fractionated by gel filtration chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.

Keywords: ESI-CID-MS/MS; fucoidan; oligosaccharide; Saccharina japonica

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