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Effects of Lissoclibadins and Lissoclinotoxins, Isolated from a Tropical Ascidian Lissoclinum cf. badium, on IL-8 production in a PMA-stimulated Promyelocytic Leukemia Cell Line
Mar. Drugs 2006, 4(1), 22-27; doi:10.3390/md401022
Article

Effects of bistheonellide A, an Actin-polymerization Inhibitor, on Chinese Hamster V79 Cells and on IL-8 Production in PMA-stimulated HL-60 Cells

1,2,* , 2, 2, 1 and 2
1 Department of Pharmaceutical Sciences, Kyoritsu University of Pharmacy, Shibakoen, Minato-ku, Tokyo 105-8512, Japan 2 Department of Ocean Sciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 108-8477, Japan
* Author to whom correspondence should be addressed.
Received: 20 January 2006 / Accepted: 21 February 2006 / Published: 21 February 2006
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Abstract

Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC50 = 6.8 nM). Bistheonellide A is an actinpolymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL- 60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system.
Keywords: Bistheonellide A; actin-polymerization inhibitor; IL-8; HL-60 cell; V79 cell Bistheonellide A; actin-polymerization inhibitor; IL-8; HL-60 cell; V79 cell
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Oda, T.; Xu, J.; Fujita, A.; Mochizuki, M.; Namikoshi, M. Effects of bistheonellide A, an Actin-polymerization Inhibitor, on Chinese Hamster V79 Cells and on IL-8 Production in PMA-stimulated HL-60 Cells. Mar. Drugs 2006, 4, 22-27.

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