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Mar. Drugs 2005, 3(2), 36-63; doi:10.3390/md302036
Article

Quality not Quantity: The Role of Marine Natural Products in Drug Discovery and Reverse Chemical Proteomics

 and *
Received: 15 April 2005 / Accepted: 30 May 2005 / Published: 1 June 2005
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Abstract

Reverse chemical proteomics combines affinity chromatography with phage display and promises to be a powerful new platform technology for the isolation of natural product receptors, facilitating the drug discovery process by rapidly linking biologically active small molecules to their cellular receptors and the receptors’ genes. In this paper we review chemical proteomics and reverse chemical proteomics and show how these techniques can add value to natural products research. We also report on techniques for the derivatisation of polystyrene microtitre plates with cleavable linkers and marine natural products that can be used in chemical proteomics or reverse chemical proteomics. Specifically, we have derivatised polystyrene with palau’amine and used reverse chemical proteomics to try and isolate the human receptors for this potent anticancer marine drug.
Keywords: palau’amine; reverse chemical proteomics; phage display palau’amine; reverse chemical proteomics; phage display
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Piggott, A.M.; Karuso, P. Quality not Quantity: The Role of Marine Natural Products in Drug Discovery and Reverse Chemical Proteomics. Mar. Drugs 2005, 3, 36-63.

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