Effect of Low Molecular Weight Oligopeptides Isolated from Sea Cucumber on Diabetic Wound Healing in db/db Mice
Abstract
:1. Introduction
2. Results
2.1. General Information
2.2. Histological Analysis
2.3. Evaluation of Inflammatory Response
2.4. Evaluation of Stromal Cell-Derived Factor-1 Alpha (SDF-1α) and NO in Serum
2.5. Evaluation of Immunostaining for Vascular Endothelial Growth Factor (VEGF)
2.6. Tensile Strength
2.7. Collagen Accumulation
2.8. Evaluation of Reactive Oxygen Species (ROS) in Serum
2.9. Albumin, Prealbumin and Transferrin Concentrations in Serum
3. Discussion
4. Materials and Methods
4.1. Preparation and Identification of SCCOPs
4.2. Animals and Groups
4.3. Incision Wound Healing Model
4.4. Measurement of Tensile Strength
4.5. Biochemical Assay
4.6. Histological Observation
4.7. Immunohistochemistry
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Time | NC | MC | WP | SCCOPs-L | SCCOPs-M | SCCOPs-H |
---|---|---|---|---|---|---|
Day 0 | 29.42 ± 1.71 | 54.87 ± 3.75 * | 53.48 ± 5.24 * | 54.64 ± 4.62 * | 55.64 ± 3.42 * | 55.57 ± 5.56 * |
Day 4 | 27.05 ± 2.04 | 49.44 ± 3.57 * | 49.23 ± 5.31 * | 49.61 ± 4.41 * | 51.03 ± 3.04 * | 50.29 ± 5.68 * |
Day 7 | 28.21 ± 2.09 | 49.77 ± 1.54 * | 47.83 ± 4.21 * | 49.80 ± 5.56 * | 49.19 ± 3.56 * | 49.79 ± 3.46 * |
Day 14 | 27.50 ± 1.58 | 50.94 ± 4.43 * | 50.45 ± 4.42 * | 48.03 ± 4.21 * | 51.92 ± 3.50 * | 48.40 ± 4.04 * |
Group | ALB (μg/L) | PA (μg/mL) | TRF (nmol/L) | ||||||
---|---|---|---|---|---|---|---|---|---|
Day 4 | Day 7 | Day 14 | Day 4 | Day 7 | Day 14 | Day 4 | Day 7 | Day 14 | |
NC | 567.07 ± 50.97 | 527.70 ± 32.51 | 568.88 ± 56.10 | 58.83 ± 2.08 | 49.65 ± 4.13 | 53.45 ± 3.35 | 281.55 ± 21.26 | 306.12 ± 17.73 | 296.91 ± 19.30 |
MC | 314.97 ± 16.11 * | 212.46 ± 23.19 * | 282.29 ± 20.40 * | 29.55 ± 1.11 * | 26.99 ± 0.82 * | 28.01 ± 2.81 * | 144.00 ± 8.85 * | 138.81 ± 14.26 * | 154.14 ± 10.60 * |
WP | 358.20 ± 5.80 * | 304.02 ± 23.91 *# | 341.04 ± 36.75 * | 35.69 ± 3.43 * | 29.97 ± 1.58 * | 33.98 ± 2.27 *# | 163.99 ± 9.49 * | 166.76 ± 12.86 *# | 170.91 ± 16.18 * |
SCCOPs-L | 331.36 ± 39.03 * | 291.97 ± 39.64 *# | 323.75 ± 23.80 * | 34.38 ± 4.09 * | 29.42 ± 2.99 * | 33.39 ± 4.41 *# | 174.97 ± 12.00 *# | 179.90 ± 11.71 *# | 183.74 ± 17.56 *# |
SCCOPs-M | 357.71 ± 29.58 * | 311.09 ± 31.00 *# | 351.37 ± 28.00 *# | 35.99 ± 3.91 *# | 35.27 ± 5.93 *# | 33.42 ± 5.38 *# | 177.39 ± 10.76 *# | 183.65 ± 12.56 *# | 186.01 ± 18.55 *# |
SCCOPs-H | 366.80 ± 21.03 * | 336.59 ± 21.85 *# | 341.50 ± 23.99 *# | 37.07 ± 3.18 *# | 36.32 ± 4.22 *# | 33.63 ± 3.61 *# | 185.16 ± 18.86 *# | 189.22 ± 17.44 *# | 190.59 ± 19.43 *# |
Amino Acid | Amino Acid Composition of SCCOPs (g/100 g) |
---|---|
Asp | 0.046 |
Glu | 0.324 |
Ser | 0.009 |
His | 0.038 |
Gly | 0.130 |
Thr | 0.249 |
Arg | 3.077 |
Ala | 0.254 |
Tyr | 0.779 |
Cys | 0.033 |
Val | 0.213 |
Met | 0.179 |
Phe | 0.593 |
Ile | 0.175 |
Leu | 0.972 |
Lys | 0.411 |
Pro | 0.015 |
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Li, D.; Li, L.; Xu, T.; Wang, T.; Ren, J.; Liu, X.; Li, Y. Effect of Low Molecular Weight Oligopeptides Isolated from Sea Cucumber on Diabetic Wound Healing in db/db Mice. Mar. Drugs 2018, 16, 16. https://doi.org/10.3390/md16010016
Li D, Li L, Xu T, Wang T, Ren J, Liu X, Li Y. Effect of Low Molecular Weight Oligopeptides Isolated from Sea Cucumber on Diabetic Wound Healing in db/db Mice. Marine Drugs. 2018; 16(1):16. https://doi.org/10.3390/md16010016
Chicago/Turabian StyleLi, Di, Lin Li, Teng Xu, Tianxing Wang, Jinwei Ren, Xinran Liu, and Yong Li. 2018. "Effect of Low Molecular Weight Oligopeptides Isolated from Sea Cucumber on Diabetic Wound Healing in db/db Mice" Marine Drugs 16, no. 1: 16. https://doi.org/10.3390/md16010016