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Mar. Drugs 2017, 15(9), 284; doi:10.3390/md15090284

Construction of a Fluorescent H2O2 Biosensor with Chitosan 6-OH Immobilized β-Cyclodextrin Derivatives

1
School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China
2
School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China
*
Author to whom correspondence should be addressed.
Received: 21 July 2017 / Revised: 18 August 2017 / Accepted: 26 August 2017 / Published: 4 September 2017
(This article belongs to the Special Issue Marine Products for Health and Beauty)
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Abstract

In the present work, a fluorescent H2O2 biosensor was constructed by encapsulating fluorescent probe Rhodamine B (RhmB) in the hydrophobic cavity of the cyclodextrin (β-CD) and immobilizing catalase (CAT) on the 2-NH2 of chitosan (CTS) in a chitosan 6-OH immobilized β-cyclodextrin derivative (CTS-6-CD). The inclusion complex of CTS-6-CD to RhmB (CTS-6-CD-RhmB) was prepared by a solution method. Its structure and inclusion efficiency were determined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and fluorescence spectroscopy (FL). CAT was immobilized on CTS-6-CD-RhmB to eventually form the functional membrane, CTS-6-CD-RhmB-CAT, via glutaraldehyde crosslinking, which was further characterized by FTIR and FL, and used as a H2O2 biosensor. The functional membrane was used to simultaneously oxidize and detect H2O2. The detection condition was optimized as pH 8, a reaction temperature of 25 °C, and an immobilized enzyme concentration of 2 × 10−4 mol/L. The fluorescence response of the biosensor exhibited a good linear relationship with the concentration of H2O2 in the range of 20 mΜ–300 μM and the detection limit of 10−8 mol/L. View Full-Text
Keywords: chitosan; catalase; β-cyclodextrin; Rhodamine B; fluorescent biosensor chitosan; catalase; β-cyclodextrin; Rhodamine B; fluorescent biosensor
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Dong, W.; Li, W.; Chen, Y.; Ye, Y.; Jin, S. Construction of a Fluorescent H2O2 Biosensor with Chitosan 6-OH Immobilized β-Cyclodextrin Derivatives. Mar. Drugs 2017, 15, 284.

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