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Mar. Drugs 2017, 15(7), 218; doi:10.3390/md15070218

Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus

1
College of Life Sciences, Qingdao University, Qingdao 266071, China
2
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
3
College of Earth Science, University of Chinese Academy of Sciences, Beijing 100049, China
4
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Anake Kijjoa
Received: 25 April 2017 / Revised: 26 June 2017 / Accepted: 5 July 2017 / Published: 10 July 2017
(This article belongs to the Special Issue Marine Products for Health and Beauty)
View Full-Text   |   Download PDF [4385 KB, uploaded 11 July 2017]   |  

Abstract

Pseudomonas stutzeri 273 is a marine bacterium producing exopolysaccharide 273 (EPS273) with high anti-biofilm activity against P. aeruginosa PAO1. Here, the complete genome of P. stutzeri 273 was sequenced and the genome contained a circular 5.03 Mb chromosome. With extensive analysis of the genome, a genetic locus containing 18 genes was predicted to be involved in the biosynthesis of EPS273. In order to confirm this prediction, two adjacent genes (eps273-H and eps273-I) encoding glycosyltransferases and one gene (eps273-O) encoding tyrosine protein kinase within the genetic locus were deleted and biosynthesis of EPS273 was checked in parallel. The molecular weight profile of EPS purified from the mutant Δeps273-HI was obviously different from that purified from wild-type P. stutzeri 273, while the corresponding EPS was hardly detected from the mutant Δeps273-O, which indicated the involvement of the proposed 18-gene cluster in the biosynthesis of EPS273. Moreover, the mutant Δeps273-HI had the biofilm formed earlier compared with the wild type, and the mutant Δeps273-O almost completely lost the ability of biofilm formation. Therefore, EPS273 might facilitate the biofilm formation for its producing strain P. stutzeri 273 while inhibiting the biofilm formation of P. aeruginosa PAO1. This study can contribute to better understanding of the biosynthesis of EPS273 and disclose the biological function of EPS273 for its producing strain P. stutzeri 273. View Full-Text
Keywords: genome; Pseudomonas stutzeri; exopolysaccharide; biosynthesis; biofilm genome; Pseudomonas stutzeri; exopolysaccharide; biosynthesis; biofilm
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Wu, S.; Zheng, R.; Sha, Z.; Sun, C. Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus. Mar. Drugs 2017, 15, 218.

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