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Mar. Drugs 2017, 15(6), 184; doi:10.3390/md15060184

Biotechnological Potential of Cold Adapted Pseudoalteromonas spp. Isolated from ‘Deep Sea’ Sponges

1
School of Microbiology, University College Cork, National University of Ireland, Cork T12 YN60, Ireland
2
Department of Research and Innovation, Matís ohf., Reykjavik 113, Iceland
3
Biomerit Research Centre, University College Cork, National University of Ireland, Cork T12 YN60, Ireland
4
School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth 6102, WA, Australia
*
Author to whom correspondence should be addressed.
Received: 22 February 2017 / Revised: 9 June 2017 / Accepted: 14 June 2017 / Published: 19 June 2017
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology II 2016)
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Abstract

The marine genus Pseudoalteromonas is known for its versatile biotechnological potential with respect to the production of antimicrobials and enzymes of industrial interest. We have sequenced the genomes of three Pseudoalteromonas sp. strains isolated from different deep sea sponges on the Illumina MiSeq platform. The isolates have been screened for various industrially important enzymes and comparative genomics has been applied to investigate potential relationships between the isolates and their host organisms, while comparing them to free-living Pseudoalteromonas spp. from shallow and deep sea environments. The genomes of the sponge associated Pseudoalteromonas strains contained much lower levels of potential eukaryotic-like proteins which are known to be enriched in symbiotic sponge associated microorganisms, than might be expected for true sponge symbionts. While all the Pseudoalteromonas shared a large distinct subset of genes, nonetheless the number of unique and accessory genes is quite large and defines the pan-genome as open. Enzymatic screens indicate that a vast array of enzyme activities is expressed by the isolates, including β-galactosidase, β-glucosidase, and protease activities. A β-glucosidase gene from one of the Pseudoalteromonas isolates, strain EB27 was heterologously expressed in Escherichia coli and, following biochemical characterization, the recombinant enzyme was found to be cold-adapted, thermolabile, halotolerant, and alkaline active. View Full-Text
Keywords: Pseudoalteromonas; enzymes; genomics; pan-genome Pseudoalteromonas; enzymes; genomics; pan-genome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Borchert, E.; Knobloch, S.; Dwyer, E.; Flynn, S.; Jackson, S.A.; Jóhannsson, R.; Marteinsson, V.T.; O’Gara, F.; Dobson, A.D.W. Biotechnological Potential of Cold Adapted Pseudoalteromonas spp. Isolated from ‘Deep Sea’ Sponges. Mar. Drugs 2017, 15, 184.

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