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Mar. Drugs 2017, 15(6), 180; doi:10.3390/md15060180

Production of Chitin from Penaeus vannamei By-Products to Pilot Plant Scale Using a Combination of Enzymatic and Chemical Processes and Subsequent Optimization of the Chemical Production of Chitosan by Response Surface Methodology

1
Grupo de Reciclado y Valorización de Materiales Residuales (REVAL), Instituto de Investigacións Mariñas (IIM-CSIC) r/Eduardo Cabello, 6, Vigo 36208, Galicia, Spain
2
Grupo de Bioquímica de Alimentos, Instituto de Investigacións Mariñas (IIM-CSIC) r/Eduardo Cabello, 6, Vigo 36208, Galicia, Spain
*
Author to whom correspondence should be addressed.
Received: 12 May 2017 / Revised: 5 June 2017 / Accepted: 9 June 2017 / Published: 16 June 2017
(This article belongs to the Special Issue Marine Chitin)
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Abstract

The waste generated from shrimp processing contains valuable materials such as protein, carotenoids, and chitin. The present study describes a process at pilot plant scale to recover chitin from the cephalothorax of Penaeus vannamei using mild conditions. The application of a sequential enzymatic–acid–alkaline treatment yields 30% chitin of comparable purity to commercial sources. Effluents from the process are rich in protein and astaxanthin, and represent inputs for further by-product recovery. As a last step, chitin is deacetylated to produce chitosan; the optimal conditions are established by applying a response surface methodology (RSM). Under these conditions, deacetylation reaches 92% as determined by Proton Nuclear Magnetic Resonance (1H-NMR), and the molecular weight (Mw) of chitosan is estimated at 82 KDa by gel permeation chromatography (GPC). Chitin and chitosan microstructures are characterized by Scanning Electron Microscopy (SEM). View Full-Text
Keywords: chitin; chitosan; Penaeus vannamei shells; response surface methodology; by-products valorization chitin; chitosan; Penaeus vannamei shells; response surface methodology; by-products valorization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Vázquez, J.A.; Ramos, P.; Mirón, J.; Valcarcel, J.; Sotelo, C.G.; Pérez-Martín, R.I. Production of Chitin from Penaeus vannamei By-Products to Pilot Plant Scale Using a Combination of Enzymatic and Chemical Processes and Subsequent Optimization of the Chemical Production of Chitosan by Response Surface Methodology. Mar. Drugs 2017, 15, 180.

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