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Mar. Drugs 2017, 15(11), 345; doi:10.3390/md15110345

Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China
University of Chinese Academy of Sciences, Beijing 100049, China
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, No. 7 Nanhai Road, Qingdao 266071, China
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, No. 61 Daizong Road, Taian 271018, China
Authors to whom correspondence should be addressed.
Received: 18 September 2017 / Revised: 29 October 2017 / Accepted: 1 November 2017 / Published: 3 November 2017
(This article belongs to the Collection Marine Polysaccharides)
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In this study, polysaccharides from Grateloupia filicinia (GFP) were extracted and several low molecular weight (Mw) G. filicina polysaccharides (LGFPs) were prepared by the hydrogen peroxide (H2O2) oxidation method. Additionally, the effect of different experimental conditions on the degradation of GFP was determined. Results showed that the GFP degradation rate was positively related to H2O2 concentration and temperature, and negatively related to pH. Chemical analysis and Fourier transform infrared spectra (FT-IR) of GFP and LGFPs showed that the degradation caused a slight decrease of total sugar and sulfate content. However, there was no obvious change for monosaccharide contents. Then, the anti-ALV-J activity of GFP and LGFPs were determined in vitro. Results revealed that all of the samples could significantly inhibit ALV-J and lower Mw LGFPs exhibited a stronger suppression, and that the fraction LGFP-3 with Mw 8.7 kDa had the best effect. In addition, the reaction phase assays showed that the inhibition effect was mainly because of the blocking virus adsorption to host cells. Moreover, real-time PCR, western-blot, and IFA were further applied to evaluate the blocking effects of LGFP-3. Results showed that the gene relative expression and gp85 protein for LGFPS-3 groups were all reduced. Data from IFA showed that there was less virus infected cells for 1000 and 200 μg/mL LGFPS-3 groups when compared to virus control. Therefore, lower Mw polysaccharides from G. filicina might supply a good choice for ALV-J prevention and treatment. View Full-Text
Keywords: Grateloupia filicina; degradation; low molecular weight polysaccharides; antiviral activity; ALV-J Grateloupia filicina; degradation; low molecular weight polysaccharides; antiviral activity; ALV-J

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Sun, Y.; Chen, X.; Cheng, Z.; Liu, S.; Yu, H.; Wang, X.; Li, P. Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J. Mar. Drugs 2017, 15, 345.

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