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Mar. Drugs 2017, 15(11), 341; https://doi.org/10.3390/md15110341

Cucumarioside A2-2 Causes Macrophage Activation in Mouse Spleen

1
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia
2
National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Science, 690041 Vladivostok, Russia
3
School of Biomedicine, Far Eastern Federal University, 690091 Vladivostok, Russia
4
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia
5
Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu City 300, Taiwan
*
Author to whom correspondence should be addressed.
Received: 30 September 2017 / Accepted: 25 October 2017 / Published: 1 November 2017
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Abstract

The immunomodulatory effect of triterpene glycoside cucumarioside A2-2 (CA2-2), isolated from the Far Eastern sea cucumber Cucumaria japonica, was compared with lipopolysaccharide (LPS) on mouse spleen. It has been shown that the intraperitoneal (i.p.) glycoside administration leads to increased spleen macrophage activating markers iba-1, IL-1β, iNOs, ROS and NO formation, with additional change of macrophage phenotype to M1. The mass spectrometry profiles of peptide/protein were obtained using MALDI-TOF-MS on the different parts of spleen sections isolated by laser mircodissection techniques. It was found that i.p. stimulation of animals with CA2-2 leads to marked changes in the intensity of the characteristic peaks of spleen peptides/proteins, primarily in red pulp. View Full-Text
Keywords: immunomodulation; LPS; iba-1; IL-1β; NO; iNOs; ROS; sea cucumbers; spleen; triterpene glycosides immunomodulation; LPS; iba-1; IL-1β; NO; iNOs; ROS; sea cucumbers; spleen; triterpene glycosides
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Pislyagin, E.A.; Manzhulo, I.V.; Gorpenchenko, T.Y.; Dmitrenok, P.S.; Avilov, S.A.; Silchenko, A.S.; Wang, Y.-M.; Aminin, D.L. Cucumarioside A2-2 Causes Macrophage Activation in Mouse Spleen. Mar. Drugs 2017, 15, 341.

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