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Mar. Drugs 2017, 15(11), 338; doi:10.3390/md15110338

Isolation and Characterization of Polysaccharides from Oysters (Crassostrea gigas) with Anti-Tumor Activities Using an Aqueous Two-Phase System

Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 8 October 2017 / Revised: 24 October 2017 / Accepted: 25 October 2017 / Published: 1 November 2017
(This article belongs to the Special Issue Marine Oligosaccharides and Polysaccharides)
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In this study, a simple aqueous two-phase system (ATPS) was employed for concurrent purification of oyster polysaccharides. The chemical structure and anti-tumor activities of purified oyster polysaccharides (OP-1) were also investigated. Under optimal ATPS conditions, oyster polysaccharides can be partitioned in the bottom phase with 67.02% extraction efficiency. The molecular weight of OP-1 was determined as 3480 Da. OP-1 is a (1→4)-α-d-glucosyl backbone and branching points located at O-3 of glucose with a terminal-d-Glcp. The anti-tumor activity assay showed that OP-1 exhibited good activities, including promotion of splenocyte proliferation, IL-2 release, and inhibition of HepG2 cell proliferation. Additionally, OP-1 had no in vivo toxicity. This finding suggests that ATPS is a much simpler and greener system, and it opens up new possibilities in the large-scale separation of active polysaccharides from oysters. OP-1 could be used by the health food and pharmaceutical therapies as potential anti-cancer adjuvants. View Full-Text
Keywords: Crassostrea gigas; polysaccharides; aqueous two-phase system; anti-tumor activity Crassostrea gigas; polysaccharides; aqueous two-phase system; anti-tumor activity

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Cheong, K.-L.; Xia, L.-X.; Liu, Y. Isolation and Characterization of Polysaccharides from Oysters (Crassostrea gigas) with Anti-Tumor Activities Using an Aqueous Two-Phase System. Mar. Drugs 2017, 15, 338.

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