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Mar. Drugs 2017, 15(11), 338; https://doi.org/10.3390/md15110338

Isolation and Characterization of Polysaccharides from Oysters (Crassostrea gigas) with Anti-Tumor Activities Using an Aqueous Two-Phase System

Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, China
These authors contributed equally to this work.
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Received: 8 October 2017 / Revised: 24 October 2017 / Accepted: 25 October 2017 / Published: 1 November 2017
(This article belongs to the Special Issue Marine Oligosaccharides and Polysaccharides)
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Abstract

In this study, a simple aqueous two-phase system (ATPS) was employed for concurrent purification of oyster polysaccharides. The chemical structure and anti-tumor activities of purified oyster polysaccharides (OP-1) were also investigated. Under optimal ATPS conditions, oyster polysaccharides can be partitioned in the bottom phase with 67.02% extraction efficiency. The molecular weight of OP-1 was determined as 3480 Da. OP-1 is a (1→4)-α-d-glucosyl backbone and branching points located at O-3 of glucose with a terminal-d-Glcp. The anti-tumor activity assay showed that OP-1 exhibited good activities, including promotion of splenocyte proliferation, IL-2 release, and inhibition of HepG2 cell proliferation. Additionally, OP-1 had no in vivo toxicity. This finding suggests that ATPS is a much simpler and greener system, and it opens up new possibilities in the large-scale separation of active polysaccharides from oysters. OP-1 could be used by the health food and pharmaceutical therapies as potential anti-cancer adjuvants. View Full-Text
Keywords: Crassostrea gigas; polysaccharides; aqueous two-phase system; anti-tumor activity Crassostrea gigas; polysaccharides; aqueous two-phase system; anti-tumor activity
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Cheong, K.-L.; Xia, L.-X.; Liu, Y. Isolation and Characterization of Polysaccharides from Oysters (Crassostrea gigas) with Anti-Tumor Activities Using an Aqueous Two-Phase System. Mar. Drugs 2017, 15, 338.

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