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Mar. Drugs 2016, 14(8), 152; doi:10.3390/md14080152

Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis

1
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
4
Hangzhou Xiaoshan Donghai Aquaculture Company Limited, Hangzhou 311200, China
*
Author to whom correspondence should be addressed.
Academic Editor: Se-Kwon Kim
Received: 28 April 2016 / Revised: 22 July 2016 / Accepted: 25 July 2016 / Published: 9 August 2016
(This article belongs to the Special Issue Marine Proteins and Peptides)
View Full-Text   |   Download PDF [10169 KB, uploaded 9 August 2016]   |  

Abstract

Anti-lipopolysaccharide factors (ALFs) with a LPS-binding domain (LBD) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. FcALF2 was one isoform of ALFs isolated from the Chinese shrimp Fenneropenaeus chinensis. Our previous study showed that a modified LBD domain (named LBDv) of FcALF2 exhibited a highly enhanced antimicrobial activity. In the present study, a modified FcALF2 gene (mFcALF2), in which the LBD was substituted by LBDv, was designed and synthesized. This gene was successfully expressed in yeast Pichia pastoris GS115 eukaryotic expression system, and the characteristics of the recombinant protein mFcALF2 were analyzed. mFcALF2 exhibited apparent antibacterial activities against Gram-negative bacteria, including Escherichia coli, Vibrio alginolyticus, Vibrio harveyi, and Vibrio parahaemolyticus, and Gram-positive bacteria, including Bacillus licheniformis and Staphylococcus epidermidis. In addition, mFcALF2 could reduce the propagation of white spot syndrome virus (WSSV) in vivo by pre-incubation with virus. The present study paves the way for developing antimicrobial drugs in aquaculture. View Full-Text
Keywords: anti-lipopolysaccharide factors; recombinant protein; antibacterial activity; antiviral activity anti-lipopolysaccharide factors; recombinant protein; antibacterial activity; antiviral activity
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MDPI and ACS Style

Yang, H.; Li, S.; Li, F.; Yu, K.; Yang, F.; Xiang, J. Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis. Mar. Drugs 2016, 14, 152.

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