Next Article in Journal
5-Episinuleptolide Decreases the Expression of the Extracellular Matrix in Early Biofilm Formation of Multi-Drug Resistant Acinetobacter baumannii
Previous Article in Journal
Marine Natural Product Inhibitors of Neutrophil-Associated Inflammation
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2016, 14(8), 142; doi:10.3390/md14080142

Sinularin Induces Apoptosis through Mitochondria Dysfunction and Inactivation of the pI3K/Akt/mTOR Pathway in Gastric Carcinoma Cells

1
Department of Food Science and Nutrition, Meiho University, Pingtung 91202, Taiwan
2
Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan
3
Antai Medical Care Cooperation Antai Tian-Sheng Memorial Hospital, Pingtung 92842, Taiwan
4
Yu Jun Biotechnology Co., Ltd., Kaoshiun 91202, Taiwan
5
Department of Nursing, Meiho University, Pingtung 91202, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Keith B. Glaser
Received: 21 March 2016 / Revised: 18 July 2016 / Accepted: 21 July 2016 / Published: 27 July 2016
View Full-Text   |   Download PDF [4281 KB, uploaded 27 July 2016]   |  

Abstract

Sinularin is an active compound isolated from the cultured soft coral Sinularia flexibilis. In this study, we investigated the effects of sinularin on two human gastric cancer cell lines, AGS and NCI-N87. Our results demonstrated that sinularin suppressed the proliferation of gastric cancer cells in a dose-dependent manner and induced apoptosis. In addition, the loss of mitochondrial membrane potential, the release of cytochrome C, the activation of Bax, Bad and caspase-3/9, and the suppression of p-Bad, Bcl-xL and Bcl-2 were observed in the cells treated with sinularin. This finding suggests that sinularin-induced apoptosis is associated with mitochondria-mediated apoptosis and occurs through caspase-dependent pathways. Furthermore, sinularin inhibited the phosphoinositol 3-kinase/Akt/mechanistic target of the rapamycin signaling pathway. Taken together, our results show that sinularin-induced apoptosis is mediated by activation of the caspase cascade and mitochondrial dysfunction. Our findings suggest that sinularin merits further evaluation as a chemotherapeutic agent for human gastric cancer. View Full-Text
Keywords: sinularin; gastric cancer; apoptosis; mitochondrial dysfunction sinularin; gastric cancer; apoptosis; mitochondrial dysfunction
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wu, Y.-J.; Wong, B.-S.; Yea, S.-H.; Lu, C.-I.; Weng, S.-H. Sinularin Induces Apoptosis through Mitochondria Dysfunction and Inactivation of the pI3K/Akt/mTOR Pathway in Gastric Carcinoma Cells. Mar. Drugs 2016, 14, 142.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top