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Mar. Drugs 2016, 14(11), 195; doi:10.3390/md14110195

Macrolactins from Marine-Derived Bacillus subtilis B5 Bacteria as Inhibitors of Inducible Nitric Oxide and Cytokines Expression

1
Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China
2
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, China
3
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, China
These authors contribute equally to this paper.
Present address: West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
*
Authors to whom correspondence should be addressed.
Academic Editor: Keith B. Glaser
Received: 25 June 2016 / Revised: 26 September 2016 / Accepted: 11 October 2016 / Published: 26 October 2016
(This article belongs to the Special Issue Deep-Sea Natural Products)
View Full-Text   |   Download PDF [1048 KB, uploaded 26 October 2016]   |  

Abstract

In order to find new natural products with anti-inflammatory activity, chemical investigation of a 3000-meter deep-sea sediment derived bacteria Bacillus subtilis B5 was carried out. A new macrolactin derivative was isolated and identified as 7,13-epoxyl-macrolactin A (1). Owing to the existence of the epoxy ring, 1 exhibited a significant inhibitory effect on the expression of inducible nitric oxide and cytokines, compared with previously isolated known macrolactins (25). Real-time Polymerase Chain Reaction (PCR) analysis showed that the new compound significantly inhibited the mRNA expressions of inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Reverse transcription-PCR analysis demonstrated that the new compound reduced the mRNA expression level of IL-1β in a concentration-dependent manner. View Full-Text
Keywords: Bacillus subtilis B5; 7,13-epoxyl-macrolactin A; macrolactins; anti-inflammatory Bacillus subtilis B5; 7,13-epoxyl-macrolactin A; macrolactins; anti-inflammatory
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MDPI and ACS Style

Yan, X.; Zhou, Y.-X.; Tang, X.-X.; Liu, X.-X.; Yi, Z.-W.; Fang, M.-J.; Wu, Z.; Jiang, F.-Q.; Qiu, Y.-K. Macrolactins from Marine-Derived Bacillus subtilis B5 Bacteria as Inhibitors of Inducible Nitric Oxide and Cytokines Expression. Mar. Drugs 2016, 14, 195.

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