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Mar. Drugs 2015, 13(8), 4934-4948; doi:10.3390/md13084934

The Marine-Derived Fungus Clonostachys rosea, Source of a Rare Conjugated 4-Me-6E,8E-hexadecadienoic Acid Reducing Viability of MCF-7 Breast Cancer Cells and Gene Expression of Lipogenic Enzymes

1
Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France
2
Faculty of Pharmacy, University of Nantes, IICiMed, 9 Rue Bias, 44000 Nantes, France
*
Author to whom correspondence should be addressed.
Academic Editor: Alejandro M. Mayer
Received: 3 June 2015 / Revised: 27 July 2015 / Accepted: 29 July 2015 / Published: 6 August 2015
(This article belongs to the Special Issue Marine Lipids)
View Full-Text   |   Download PDF [364 KB, uploaded 7 August 2015]   |  

Abstract

A marine-derived strain of Clonostachys rosea isolated from sediments of the river Loire estuary (France) was investigated for its high lipid production. The fungal strain was grown on six different culture media to explore lipid production changes. An original branched conjugated fatty acid, mainly present in triglycerides and mostly produced when grown on DCA (23% of total fatty acid composition). It was identified as 4-Me-6E,8E-hexadecadienoic on the basis of spectroscopic analyses. This fatty acid reduced viability of MCF-7 breast cancer cells in a dose dependent manner (up to 63%) at physiological free fatty acid human plasma concentration (100 μM). Reduction of gene expression of two lipogenic enzymes, the acetyl CoA carboxylase (ACC) and the fatty acid synthase (FAS) was evaluated to explore the mechanisms of action of 4-Me-6E,8E-16:2 acid. At 50 μM, 50% and 35% of mRNA gene expression inhibition were observed for ACC and FAS, respectively. View Full-Text
Keywords: Clonostachys rosea; fatty acids; conjugated fatty acid; lipids; cancer; anti-carcinogenic; MCF-7; lipogenesis; acetyl-CoA carboxylase; fatty acid synthase Clonostachys rosea; fatty acids; conjugated fatty acid; lipids; cancer; anti-carcinogenic; MCF-7; lipogenesis; acetyl-CoA carboxylase; fatty acid synthase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Dias, A.C.D.S.; Ruiz, N.; Couzinet-Mossion, A.; Bertrand, S.; Duflos, M.; Pouchus, Y.-F.; Barnathan, G.; Nazih, H.; Wielgosz-Collin, G. The Marine-Derived Fungus Clonostachys rosea, Source of a Rare Conjugated 4-Me-6E,8E-hexadecadienoic Acid Reducing Viability of MCF-7 Breast Cancer Cells and Gene Expression of Lipogenic Enzymes. Mar. Drugs 2015, 13, 4934-4948.

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