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Mar. Drugs 2015, 13(6), 3791-3808; doi:10.3390/md13063791

Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains

1
Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona 08071, Spain
2
Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Antonio Molinaro
Received: 14 April 2015 / Accepted: 27 April 2015 / Published: 15 June 2015
(This article belongs to the Special Issue Marine Lipopolysaccharides)
View Full-Text   |   Download PDF [1082 KB, uploaded 15 June 2015]   |  

Abstract

The A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now, only repeating units with one or two different monosaccharides have been described. Functional genomic analysis of this wbsalmo region is mostly in agreement with the LPS O-antigen structure of acetylated l-rhamnose (Rha), d-glucose (Glc), and 2-amino-2-deoxy-d-mannose (ManN). Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains). Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer. A. salmonicida subsp. pectinolytica strains lack wbsalmo and A-layer, two major virulence factors, and this could be the reason they are the only ones not found as fish pathogens. View Full-Text
Keywords: Aeromonas salmonicida; subspecies; genomics; proteomics; lipopolysaccharide O-antigen; A-surface layer Aeromonas salmonicida; subspecies; genomics; proteomics; lipopolysaccharide O-antigen; A-surface layer
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Merino, S.; de Mendoza, E.; Canals, R.; Tomás, J.M. Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains. Mar. Drugs 2015, 13, 3791-3808.

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