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Mar. Drugs 2015, 13(5), 3241-3258; doi:10.3390/md13053241

Enhanced Control of Bladder-Associated Tumors Using Shrimp Anti-Lipopolysaccharide Factor (SALF) Antimicrobial Peptide as a Cancer Vaccine Adjuvant in Mice

1
Department of Food Science, National Taiwan Ocean University, Keelung 202, Taiwan
2
Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Road, Jiaushi, Ilan 262, Taiwan
3
Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung 811, Taiwan
4
Department of Life Science, Chinese Culture University, Taipei 111, Taiwan
*
Authors to whom correspondence should be addressed.
Academic Editors: Peter Duggan and Kellie L. Tuck
Received: 9 March 2015 / Accepted: 12 May 2015 / Published: 21 May 2015
(This article belongs to the Special Issue Marine Peptides and Their Mimetics)
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Abstract

Shrimp anti-lipopolysaccharide factor (SALF) is an antimicrobial peptide with reported anticancer activities, such as suppression of tumor progression. In this study, we prepared a potential cancer vaccine comprised of SALF in conjunction with the cell lysate of inactivated murine bladder carcinoma cells (MBT-2), and evaluated its efficacy in a mouse tumor model. Our study shows that SALF added to cell culture media inhibits growth progression of MBT-2, and that SALF together with inactivated MBT-2 lysate elevates the level of inflammasome activity, and modulates the levels of IL-1β, MCP-1, IL-6, IL-12, and TNF-α in mouse macrophages. Immunization of 7, 14, and 21 day-old mice with the vaccine prevented growth of MBT-2 cell-mediated tumors. The vaccine was found to enhance expression of T-cell, cytotoxic T cells, and NK cells in the immunized mice groups. Recruitment of macrophages, T-helper cells, and NK cells was enhanced, but levels of VEGF were decreased in immunized mice. This report provides empirical evidence that our SALF as vaccine adjuvant enhances antitumor immunity in mice. View Full-Text
Keywords: SALF; antimicrobial peptide; MBT-2; bladder tumor; cancer vaccine SALF; antimicrobial peptide; MBT-2; bladder tumor; cancer vaccine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Huang, H.-N.; Rajanbabu, V.; Pan, C.-Y.; Chan, Y.-L.; Chen, J.-Y.; Wu, C.-J. Enhanced Control of Bladder-Associated Tumors Using Shrimp Anti-Lipopolysaccharide Factor (SALF) Antimicrobial Peptide as a Cancer Vaccine Adjuvant in Mice. Mar. Drugs 2015, 13, 3241-3258.

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