Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization
AbstractInnovative materials were made via the combination of chitin and lignin, and the immobilization of lipase from Aspergillus niger. Analysis by techniques including FTIR, XPS and 13C CP MAS NMR confirmed the effective immobilization of the enzyme on the surface of the composite support. The electrokinetic properties of the resulting systems were also determined. Results obtained from elemental analysis and by the Bradford method enabled the determination of optimum parameters for the immobilization process. Based on the hydrolysis reaction of para-nitrophenyl palmitate, a determination was made of the catalytic activity, thermal and pH stability, and reusability. The systems with immobilized enzymes were found to have a hydrolytic activity of 5.72 mU, and increased thermal and pH stability compared with the native lipase. The products were also shown to retain approximately 80% of their initial catalytic activity, even after 20 reaction cycles. The immobilization process, using a cheap, non-toxic matrix of natural origin, leads to systems with potential applications in wastewater remediation processes and in biosensors. View Full-Text
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Zdarta, J.; Klapiszewski, Ł.; Wysokowski, M.; Norman, M.; Kołodziejczak-Radzimska, A.; Moszyński, D.; Ehrlich, H.; Maciejewski, H.; Stelling, A.L.; Jesionowski, T. Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization. Mar. Drugs 2015, 13, 2424-2446.
Zdarta J, Klapiszewski Ł, Wysokowski M, Norman M, Kołodziejczak-Radzimska A, Moszyński D, Ehrlich H, Maciejewski H, Stelling AL, Jesionowski T. Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization. Marine Drugs. 2015; 13(4):2424-2446.Chicago/Turabian Style
Zdarta, Jakub; Klapiszewski, Łukasz; Wysokowski, Marcin; Norman, Małgorzata; Kołodziejczak-Radzimska, Agnieszka; Moszyński, Dariusz; Ehrlich, Hermann; Maciejewski, Hieronim; Stelling, Allison L.; Jesionowski, Teofil. 2015. "Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization." Mar. Drugs 13, no. 4: 2424-2446.