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Correction published on 9 September 2015, see Mar. Drugs 2015, 13(9), 5732-5735.

Open AccessArticle
Mar. Drugs 2015, 13(3), 1317-1339; doi:10.3390/md13031317

Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum

Laboratory of Plant and Cell Physiology/Laboratoire de Physiologie Cellulaire et Végétale, Unité mixte de recherche 5168 CNRS-CEA-Université Grenoble Alpes, Institut de Recherche en Sciences et Technologies pour le Vivant, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
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Author to whom correspondence should be addressed.
Academic Editor: Véronique Martin-Jézéquel
Received: 17 December 2014 / Revised: 17 February 2015 / Accepted: 28 February 2015 / Published: 16 March 2015
(This article belongs to the Special Issue Metabolites in Diatoms)
View Full-Text   |   Download PDF [1178 KB, uploaded 17 March 2015]   |  

Abstract

The diatom Phaeodactylum is rich in very long chain polyunsaturated fatty acids (PUFAs). Fatty acid (FA) synthesis, elongation, and desaturation have been studied in depth in plants including Arabidopsis, but for secondary endosymbionts the full picture remains unclear. FAs are synthesized up to a chain length of 18 carbons inside chloroplasts, where they can be incorporated into glycerolipids. They are also exported to the ER for phospho- and betaine lipid syntheses. Elongation of FAs up to 22 carbons occurs in the ER. PUFAs can be reimported into plastids to serve as precursors for glycerolipids. In both organelles, FA desaturases are present, introducing double bonds between carbon atoms and giving rise to a variety of molecular species. In addition to the four desaturases characterized in Phaeodactylum (FAD2, FAD6, PtD5, PtD6), we identified eight putative desaturase genes. Combining subcellular localization predictions and comparisons with desaturases from other organisms like Arabidopsis, we propose a scheme at the whole cell level, including features that are likely specific to secondary endosymbionts. View Full-Text
Keywords: Arabidopsis; phaeodactylum; diatoms; fatty acid; desaturase; lipid; EPA; front-end desaturase; polyunsaturated fatty acids Arabidopsis; phaeodactylum; diatoms; fatty acid; desaturase; lipid; EPA; front-end desaturase; polyunsaturated fatty acids
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Dolch, L.-J.; Maréchal, E. Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317-1339.

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