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Mar. Drugs 2015, 13(1), 102-127; doi:10.3390/md13010102

Chemical Profiling (HPLC-NMR & HPLC-MS), Isolation, and Identification of Bioactive Meroditerpenoids from the Southern Australian Marine Brown Alga Sargassum paradoxum

School of Applied Sciences (Discipline of Chemistry), Health Innovations Research Institute (HIRi) RMIT University, GPO Box 2476V Melbourne, Victoria 3001, Australia
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Author to whom correspondence should be addressed.
Academic Editor: Orazio Taglialatela-Scafati
Received: 7 November 2014 / Accepted: 15 December 2014 / Published: 29 December 2014
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Abstract

A phytochemical investigation of a southern Australian marine brown alga, Sargassum paradoxum, resulted in the isolation and identification of four new (5, 9, 10, and 15) and nine previously reported (1, 2, 68, and 1114) bioactive meroditerpenoids. HPLC-NMR and HPLC-MS were central to the identification of a new unstable compound, sargahydroquinal (9), and pivotal in the deconvolution of eight (1, 2, 57, and 1012) other meroditerpenoids. In particular, the complete characterization and identification of the two main constituents (1 and 2) in the crude dichloromethane extract was achieved using stop-flow HPLC-NMR and HPLC-MS. This study resulted in the first acquisition of gHMBCAD NMR spectra in the stop-flow HPLC-NMR mode for a system solely equipped with a 60 μL HPLC-NMR flow cell without the use of a cold probe, microcoil, or any pre-concentration. View Full-Text
Keywords: Sargassum; profiling; dereplication; HPLC-NMR; HPLC-MS; bioassay-guided; biological activity; meroditerpenoid Sargassum; profiling; dereplication; HPLC-NMR; HPLC-MS; bioassay-guided; biological activity; meroditerpenoid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Brkljača, R.; Urban, S. Chemical Profiling (HPLC-NMR & HPLC-MS), Isolation, and Identification of Bioactive Meroditerpenoids from the Southern Australian Marine Brown Alga Sargassum paradoxum. Mar. Drugs 2015, 13, 102-127.

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