Mar. Drugs 2014, 12(5), 2341-2356; doi:10.3390/md12052341
Article

Omega-3 Polyunsaturated Fatty Acids Protect Neural Progenitor Cells against Oxidative Injury

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Received: 7 February 2014; in revised form: 26 March 2014 / Accepted: 3 April 2014 / Published: 29 April 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The omega-3 polyunsaturated fatty acids (ω-3 PUFAs), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), derived mainly from fish oil, play important roles in brain development and neuroplasticity. Here, we reported that application of ω-3 PUFAs significantly protected mouse neural progenitor cells (NPCs) against H2O2-induced oxidative injury. We also isolated NPCs from transgenic mice expressing the Caenorhabditis elegans fat-1 gene. The fat-1 gene, which is absent in mammals, can add a double bond into an unsaturated fatty acid hydrocarbon chain and convert ω-6 to ω-3 fatty acids. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining showed that a marked decrease in apoptotic cells was found in fat-1 NPCs after oxidative injury with H2O2 as compared with wild-type NPCs. Quantitative RT-PCR and Western blot analysis demonstrated a much higher expression of nuclear factor erythroid 2-related factor 2 (Nrf2), a master transcriptional factor for antioxidant genes, in fat-1 NPCs. The results of the study provide evidence that ω-3 PUFAs resist oxidative injury to NPCs.
Keywords: oxidative stress; DHA; fat-1; neural progenitor cells
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MDPI and ACS Style

Liu, Q.; Wu, D.; Ni, N.; Ren, H.; Luo, C.; He, C.; Kang, J.-X.; Wan, J.-B.; Su, H. Omega-3 Polyunsaturated Fatty Acids Protect Neural Progenitor Cells against Oxidative Injury. Mar. Drugs 2014, 12, 2341-2356.

AMA Style

Liu Q, Wu D, Ni N, Ren H, Luo C, He C, Kang J-X, Wan J-B, Su H. Omega-3 Polyunsaturated Fatty Acids Protect Neural Progenitor Cells against Oxidative Injury. Marine Drugs. 2014; 12(5):2341-2356.

Chicago/Turabian Style

Liu, Qiang; Wu, Di; Ni, Na; Ren, Huixia; Luo, Chuanming; He, Chengwei; Kang, Jing-Xuan; Wan, Jian-Bo; Su, Huanxing. 2014. "Omega-3 Polyunsaturated Fatty Acids Protect Neural Progenitor Cells against Oxidative Injury." Mar. Drugs 12, no. 5: 2341-2356.


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