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A correction was published on 5 September 2014, see Mar. Drugs 2014, 12(9), 4741-4742.

Mar. Drugs 2014, 12(3), 1298-1316; doi:10.3390/md12031298
Article

Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16

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Received: 24 December 2013; in revised form: 27 January 2014 / Accepted: 8 February 2014 / Published: 7 March 2014
(This article belongs to the Special Issue Marine Lipopolysaccharides)
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Abstract: Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16. ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep5Hex3HexN1Kdo1P1. Sugar and methylation analysis together with 1D and 2D 1H and 13C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-d-Quip3NAc-(1→3)-α-l-Rhap-(1→4)-β-d-Galp-(1→3)-α-d-GalpNAc-(1→. This composition was confirmed by mass spectrometry. The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H2O). Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
Keywords: lipopolysaccharide; O-specific polysaccharide; Aeromonas veronii; fish pathogen; ESI MS; NMR lipopolysaccharide; O-specific polysaccharide; Aeromonas veronii; fish pathogen; ESI MS; NMR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Turska-Szewczuk, A.; Duda, K.A.; Schwudke, D.; Pekala, A.; Kozinska, A.; Holst, O. Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16. Mar. Drugs 2014, 12, 1298-1316.

AMA Style

Turska-Szewczuk A, Duda KA, Schwudke D, Pekala A, Kozinska A, Holst O. Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16. Marine Drugs. 2014; 12(3):1298-1316.

Chicago/Turabian Style

Turska-Szewczuk, Anna; Duda, Katarzyna A.; Schwudke, Dominik; Pekala, Agnieszka; Kozinska, Alicja; Holst, Otto. 2014. "Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16." Mar. Drugs 12, no. 3: 1298-1316.


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