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Mar. Drugs 2014, 12(10), 5240-5257; doi:10.3390/md12105240

Antimicrobial Activity of Peptides Derived from Olive Flounder Lipopolysaccharide Binding Protein/Bactericidal Permeability-Increasing Protein (LBP/BPI)

1
Biotechnology Research Division, National Fisheries Research and Development Institute, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea
2
Department of Biotechnology, Pukyong National University, Busan 608-737, Korea
3
Department of Food Science and Biotechnology, Kunsan National University, Korea
*
Authors to whom correspondence should be addressed.
Received: 25 July 2014 / Revised: 18 September 2014 / Accepted: 7 October 2014 / Published: 17 October 2014
(This article belongs to the Special Issue Marine Peptides and Their Mimetics)
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Abstract

We describe the antimicrobial function of peptides derived from the C-terminus of the olive flounder LBP BPI precursor protein. The investigated peptides, namely, ofLBP1N, ofLBP2A, ofLBP4N, ofLBP5A, and ofLBP6A, formed α-helical structures, showing significant antimicrobial activity against several Gram-negative bacteria, Gram-positive bacteria, and the yeast Candida albicans, but very limited hemolytic activities. The biological activities of these five analogs were evaluated against biomembranes or artificial membranes for the development of candidate therapeutic agents. Gel retardation studies revealed that peptides bound to DNA and inhibited migration on an agarose gel. In addition, we demonstrated that ofLBP6A inhibited polymerase chain reaction. These results suggested that the ofLBP-derived peptide bactericidal mechanism may be related to the interaction with intracellular components such as DNA or polymerase. View Full-Text
Keywords: antimicrobial peptide; LPS-binding protein; bactericidal permeability-increasing protein; analogs; antimicrobial mechanism antimicrobial peptide; LPS-binding protein; bactericidal permeability-increasing protein; analogs; antimicrobial mechanism
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nam, B.-H.; Moon, J.-Y.; Park, E.-H.; Kim, Y.-O.; Kim, D.-G.; Kong, H.J.; Kim, W.-J.; Jee, Y.J.; An, C.M.; Park, N.G.; Seo, J.-K. Antimicrobial Activity of Peptides Derived from Olive Flounder Lipopolysaccharide Binding Protein/Bactericidal Permeability-Increasing Protein (LBP/BPI). Mar. Drugs 2014, 12, 5240-5257.

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