- freely available
Mar. Drugs 2013, 11(7), 2486-2500; doi:10.3390/md11072486
Published: 15 July 2013
Abstract: Chemical interactions play a fundamental role in the ecology of marine foodwebs. Dimethyl sulfide (DMS) is a ubiquitous marine trace gas that acts as a bioactive compound by eliciting foraging behavior in a range of marine taxa including the copepod Temora longicornis. Production of DMS can rapidly increase following microzooplankton grazing on phytoplankton. Here, we investigated whether grazing-induced DMS elicits an increase in foraging behavior in the copepod Calanus helgolandicus. We developed a semi-automated method to quantify the effect of grazing-mediated DMS on the proportion of the time budget tethered females allocate towards slow swimming, typically associated with feeding. The pooled data showed no differences in the proportion of the 25 min time budget allocated towards slow swimming between high (23.6 ± 9.74%) and low (29.1 ± 18.33%) DMS treatments. However, there was a high degree of variability between behavioral responses of individual copepods. We discuss the need for more detailed species-specific studies of individual level responses of copepods to chemical signals at different spatial scales to improve our understanding of chemical interactions between copepods and their prey.
Interactions mediated by bioactive infochemicals operate across a range of temporal and spatial scales in marine environments, influencing population dynamics, community structure and ecosystem functioning . These bioactive compounds affect fundamental and diverse processes including food selectivity, prey encounter and capture, mating interactions, chemical defense, behavior and population synchronization . The climate-relevant trace gas dimethyl sulfide (DMS) is formed following the cleavage of the temporally abundant algal secondary metabolite dimethylsulfoniopropionate (DMSP). Several studies have indicated that airborne DMS from marine plankton is a bioactive compound that acts as a directional foraging cue to a range of vertebrate predators enabling them to locate high productivity prey patches [3,4,5,6]. The process of DMS-cleavage is accelerated following viral lysis, natural senescence and grazing. Steinke et al.  showed that the copepod Temora longicornis can detect gradients of DMS and responds with a change in its swimming behavior. Since copepods can effectively regulate phytoplankton and microzooplankton populations (e.g., [8,9]) their ability to react to grazing-induced DMS could be an important process in structuring pelagic ecosystems. Ubiquitous in the marine environment, volatile DMS provides an ideal model for investigating chemically-mediated trophic interactions in plankton communities . Recently, Ferrer and Zimmer  suggested that DMSP and DMS are “molecules of keystone significance”, which, analogous to the keystone species concept, have the potential to initiate major trophic cascades and structure marine communities. This is supported by mathematical simulations that suggest that DMS and other infochemicals are potentially important in structuring planktonic multitrophic interactions . Despite the potential ecological significance of bioactive chemical interactions structuring planktonic communities, the behavioral responses of planktonic predators to grazing-induced volatiles remains poorly resolved [2,12].
Phytoplankton succession leads to small DMS-producing species dominating phytoplankton assemblages during the summer . Copepods, such as Calanus helgolandicus, feed inefficiently on small phytoplankton  and preferentially prey on microzooplankton, which form a crucial trophic bridge between primary producers and higher trophic levels . Grazing by microzooplankton on DMSP-containing phytoplankton has been shown to result in rapid production of DMS both in laboratory and field experiments [16,17]. Under such conditions, DMS has the potential to mediate trophic interactions across planktonic trophic levels. Copepods display considerable behavioral plasticity in response to chemical and hydromechanical stimulation, and these behaviors may be modified or habituate with time after initial exposure [18,19]. The ability for copepods to detect and modify their behavior in response to grazing-induced DMS production may enhance their foraging success.
The copepod Calanus helgolandicus employs a “hop and sink” swimming strategy involving a period of swimming followed by a period of inactivity when the copepod sinks. These predominant behaviors are interspersed with rapid grooming events and escape reactions . During slow swimming C. helgolandicus generate a large laminar feeding current drawing prey towards the mouth appendages. Maintenance of the copepod feeding currents is not considered to be energetically expensive [21,22], but in the absence of prey stimuli, breaking will allow negatively buoyant copepods to sink slowly through the water column until further detection of prey. As generation of the feeding current causes hydrodynamic disturbances to a volume of water up to 180 times the volume of the copepod [23,24], breaking in the absence of prey cues allows copepods to remain less conspicuous to potential predators. As such, copepod behaviour is a trade-off between optimising encounters with prey whilst reducing energetic costs and susceptibility to predation [25,26]. Such behavioral trade-offs are likely linked to perceived prey density and the perceived threat of predation .
Detection of copepod responses to prey stimuli has previously been measured in two ways, including through changes in the frequency of appendage beating or the proportion of the time budget allocated towards slow swimming. Gill and Harris  observed significant differences in the beat frequency of the first maxilla in C. helogolandicus in response to dinoflagellate and diatom diets in comparison to seawater controls, but the observed differences were typically small. Conversely, large differences in the proportion of the time budget spent slow swimming were observed between some dinoflagellate diets (Prorocentrum micans 11.5% and Scrippsiella trochoidea 28.4%) and the diatom treatment (Thalassiosira weissflogii 38.9%). However, variation in the proportion of the time budget spent slow swimming was high . Here, we used a semi-automated technique to test the hypothesis that DMS generated following microzooplankton grazing increases the amount of time copepods spend slow swimming. We quantified the behavior of tethered C. helgolandicus feeding on the heterotrophic dinoflagellate Oxyrrhis marina that was pre-conditioned with either a high or low DMS-producing strain of the phytoplankton Emiliania huxleyi.
2. Experimental Section
Two non-calcifying strains of the coccolithophore Emiliania huxleyi, a high DMS-producing strain (CCMP 373) and a low DMS-producing strain (CCMP370) were purchased from the National Centre for Marine Algae and Microbiota (NCMA) and maintained in batch culture in ESAW medium  at 15 °C and a light intensity of 100 μmol m−2 s−1 during a 12 h light: 12 h dark cycle. The heterotrophic dinoflagellate Oxyrrhis marina (CCAP 1133/5) was cultured in filtered (0.2 μm poresize) and autoclaved seawater (FASW), and maintained on a diet of non-DMS producing Dunaliella tertiolecta (grown in ESAW as above). Following grazing by O. marina, CCMP 373 produces 15-fold higher DMS concentrations than CCMP 370 . Prior to experiments, O. marina was placed in the dark for 24 h to eliminate prey. Copepods (Calanus helgolandicus) were collected weekly by trawling with a coarse mesh cod-end at the Western Channel Observatory sampling site L4 off Plymouth, U.K. (50°15′ N, 04°13′ W). Copepods were transported to the laboratory in insulated cool boxes before adult females (average prosome length = 2.21 ± 0.119 mm) were microscopically identified and transferred into 20 L buckets containing FASW in a dark walk-in room at 15 °C. They were maintained on a diet of Rhodomonas salina and O. marina at approximately 200 μg C L−1.
Adult females were tethered to a hair 24 h prior to experiments. Hairs were treated with acetone and washed with Milli-Q water to remove natural odors. The hair was glued to the end of a glass Pasteur pipette which had been heat molded into a position allowing copepods to be oriented in a natural swimming position. During tethering, copepods were placed ventral side down in a concaved microscope slide with the feeding appendages and antennae immersed in water. The dorsal prosome was gently dabbed dry with microscope cleaning tissue and the hair glued to its base using instant adhesive (3M Scotch-Weld CA4OH). Tethered copepods were transferred into filtered seawater and fed O. marina. Tethered individuals typically survived for several weeks and copepods used in experiments were maintained for a minimum of 24 h after the experiment to ensure survival before freezing at −80 °C for storage and body measurements.
2.3. Filming Set-Up
Filming was carried out in a dark controlled-temperature room at 15 °C. Copepods were attached to a micromanipulator and placed in 1 L polycarbonate bottles filled to the neck with FASW. A CCTV camera (Watec, WAT-902H) with a 60 mm macro lens (Nikkor) was connected to a computer to record copepod behavior. Illumination was provided using collimated light from an infrared light-emitting diode (peak wavelength 940 nm).
Polycarbonate bottles were inoculated with either E. huxleyi CCMP370 (low DMS) or CCMP373 (high DMS) to achieve a density of 6000 cells mL−1. Oxyrrhis marina was added to achieve a final density of 350 cells mL−1. Bottles were placed on a plankton wheel at 1 rpm and incubated for 5 h to allow grazing interactions to occur and the build-up of DMS. A total of 16 copepods were exposed to each treatment for 45 min using the following treatments in sequence: FASW, initial treatment diet, FASW, alternate treatment diet. The order of treatment (low DMS and high DMS diet) was randomized to prevent potential bias. The experimental design was based around a repeated measures approach allowing comparisons of individual copepods behavior between high and low DMS treatments.
2.5. DMS Quantification
DMS measurements were taken from 5 mL samples, gravity filtered through a 25 mm GF/F filter into a narrow glass vial minimizing diffusive losses. Depending on the expected DMS concentration in the sample, 1 to 4 mL was immediately transferred from the bottom of the vial to a purge-and-trap system for cryogenic enrichment of DMS before quantification using gas chromatography (Varian 3800) with a pulsed flame photometric detector (PFPD) . Each experimental replicate was sampled in analytical duplicate.
2.6. Behavioral Analysis
A semi-automated technique was developed to assign copepod behavior into three states: breaking, slow swimming or fast swimming (including escape reactions and grooming see Figure 1 and supplementary material). A custom MATLAB code subtracted the pixel intensities of two consecutive grey-scale video frames and determined the maximum change in pixel intensity, δi, across the matrix obtained from this subtraction. Since copepods were tethered, no movement (breaking) led to small changes in pixel intensity, continuous rhythmic feeding appendage movements (slow swimming) led to intermediate changes in pixel intensity and rapid movements of the body (fast swimming) led to large values in δi. Subsequent analysis of δi time series was conducted in the R programming language . To avoid erroneous classifications due to short-term fluctuations in δi, we smoothed these time series by averaging δi over a five-frame window. By visual comparison of the δi time series to observed behavior in our video recordings, we determined two thresholds for each film: one to distinguish breaking from swimming and another to distinguish slow from fast swimming (Figure 2). Using these thresholds, we then converted the δi time series into time series of behavioral states adopted by the copepods. To ensure the robustness of this approach, we visually compared the automated classification for each film to the behavioral states observed in our video recordings at three time points over 1000 frames at the beginning, middle and towards the end of the video sequences. In order to gain an estimation of error, the automated method was compared to a previous data set that had been analyzed manually frame-by-frame (see results). Of the total 16 copepods filmed, the data from four copepods were discarded due to difficulties in assigning behavioral threshold values in at least one of the treatment videos. The behavioral state of 12 copepods was extracted for a period of 25 min from the beginning of recordings. Analysis over this period of time (37,500 frames) allows observation of behaviors at the onset of each treatment and is sufficiently long enough to allow copepods to habituate to the treatment conditions . Due to the time taken to position the copepod and focus the camera system between replicates, analysis commenced approximately 1 min after initial transfer. Behavioral states were recorded at a temporal resolution of 25 frames s−1.
2.7. Statistical Analyses
Percentage data on the copepod behavioral time budgets was arcsine transformed and tested for normality. t-tests were initially used to determine if there were any differences in the average duration of time spent slow swimming in each treatment depending on the order they were received. The effect of DMS treatment and time was tested by analyzing each minute of behavioral data for the 12 copepods over the 25 min time interval of the experiment using 2-way repeated measures analysis of variance in SPSS Statistics 18 setting the threshold for α at 0.05. Differences in the proportion of the time budget allocated towards slow swimming between the two treatments were also tested at the individual level. The difference between the means of paired observations for slow swimming percentage over the 25 min time interval were compared by testing confidence intervals with matched pairs analysis for small sample sizes .
3. Results and Discussion
3.1. Validation of Behavioral Analysis
A semi-automated approach was developed to test the hypothesis that grazing-mediated DMS production would stimulate an increase in the proportion of time Calanus helgolandicus spent feeding. We used three videos from a previous study  to verify the semi-automated analysis of copepod swimming behavior. Each video was 15 min in length and each of the total 67,500 frames had been manually scored for the different behavior types. The semi-automated approach consistently overestimated the proportion of the time budget allocated to slow swimming by an average of 3.7 ± 0.48% of the total time. This error may be due to the necessary smoothing of data and was considered acceptable given the significant decrease in time required for analysis. Since improvements of our optical set-up have been made since the previous study, improved image resolution will have resulted in an estimated error of <3.7% in our analysis of slow swimming behavior. The method allowed efficient analysis of tethered copepod behavior at a high temporal resolution for extended video sequences compared to laborious manual analysis by eye. A disadvantage of the method was that all “fast swimming” behaviors were pooled, as escape reactions and grooming events could not be differentiated in our analysis. However, these behaviors typically accounted for less than 1% of the behavioral time budget of all copepods in our experiments.
3.2. DMS Quantification
Grazing by Oxyrrhis marina on the low DMS-producing strain of Emiliania huxleyi (CCMP370)resulted in concentrations of 1.8 ± 0.67 nM DMS in the low DMS treatments after 5 h incubation. Grazing by O. marina on the high DMS-producing strain of E. huxleyi (CCMP373) in the high DMS treatment resulted in significantly higher DMS production with final concentrations of 13.1 ± 4.30 nM (t = 13.46 d.f. 21, p < 0.001). Filtered seawater used in the experiments had a natural DMS concentration of 1.2 nM (±0.75).
3.3. Pooled Copepod Behavior
Slow swimming and breaking behaviors accounted for the majority of the copepod behavioral repertoire. Fast swimming behaviors, including escape reactions and grooming events, made up the remaining time budget and accounted for less than 1% of the 25 min time budget across all copepods and treatments. Treatment order did not influence the average time spent slow swimming during the 25 min period of analysis. Copepods spent the same proportion of the total time budget slow swimming irrespective of the order they received high (t1,10 = 0.44, p = 0.667) or low DMS treatments (t1,10 = 0.10, p = 0.924). There were no significant differences in the average proportion of the 25 min time budget the 12 copepods allocated towards slow swimming in the high (23.6 ± 9.74%) and low (29.1 ± 18.33%) DMS treatments (Figure 3, Table 1). The behavior of the copepods changed significantly with time throughout the duration of the 25-min experiments in both treatments (Table 1), with pooled data showing that copepods spent more time slow swimming at the onset of each treatment (Figure 3). Copepods have previously been shown to increase swimming and escape reactions immediately in response to treatment perturbations but such behaviors can be reduced through treatment habituation during the 25 min treatment and filming [18,19]. In our experiments there was no significant interaction effect between DMS treatment and time (Table 1).
|Table 1. Results of a repeated measures ANOVA test showing the effect of high and low DMS treatments, and time on the proportion of the time budget copepods spent slow swimming. The behavior of 12 copepods was analyzed for 25 min, with each minute being tested in the model.|
|DMS × Time||24||0.015||1.45||0.087|
|Error (DMS × Time)||240||0.100|
3.4. Individual Copepod Behavior
Individual copepods varied in their behavioral responses at the onset of each treatment (Figure 4). Matched pairs analysis showed that three of the twelve individuals (copepods 1–3) spent significantly more time slow swimming in high DMS treatments, whereas six copepods (7–12) spent significantly more time slow swimming in low DMS treatments (Figure 4). Differences in the proportion of the time budget dedicated to slow swimming between the mean of matched pairs analysis from the high and low DMS treatments was checked against the differences in measured DMS concentration between the paired treatments but no trend was observed (data not shown). Whilst there were no clear trends in the proportion of time spent slow swimming by C. helgolandicus in response to DMS, a large degree of variability in the time budget allocated towards slow swimming was observed between copepods at the individual level irrespective of DMS concentration (Figure 4). For example, Copepod 7 spent a large proportion of the total time budget slow swimming in the low DMS treatments (72.5%) whilst Copepod 3 spent just 3.9% of the time budget slow swimming in the same treatment. Whilst it is important to consider that the results from these experiments are drawn from a sample size of 12 copepods and are subject to experimental noise, they do raise questions regarding the variability of behavior. An interesting avenue for future studies is to determine the potential existence of individual “personalities”, as described for invertebrate behaviors  and recently revealed in decapod crustaceans . Such differences in individual behavior within a population may diminish the effects of changes to the environment as not all individuals react in the same fashion . Future studies should consider repeated exposure of the same individual to a particular experimental treatment to provide more detailed insight into the degree of behavioral variation in ecologically important copepods.
Pelagic copepods are taxonomically diverse and display various swimming and feeding strategies. A range of environmental cues transcending various scales are utilized to maximize copepod foraging success . The relative importance of bioactive compounds relative to other cues in determining copepod foraging success is likely to be constrained by species-specific feeding ecology. From the perspective of a foraging copepod, bioactive infochemicals such as DMS, potentially operate across three broadly defined scales: (1) the micro or cellular scale where cellular exudates may provide copepods with an advanced warning of an approaching prey item; (2) the meso or patch scale where background chemical cues could elicit feeding associated behavior modifications; and (3) the macro or water column scale where chemical gradients may provide directional cues to locate high productivity ephemeral prey patches. Our experiments exposed C. helgolandicus to a productive prey patch with either a high or low DMS signal. In such a scenario at least two of the above mechanisms could operate to provide copepods with chemical foraging cues. Below we discuss our results in light of previous findings on the response of copepods to chemical cues and in relation to species-specific feeding ecology and ecologically-relevant scales.
The ability of copepods to utilize chemical cues at the microscale is highly constrained by swimming strategy. For example, ambush predators such as Oithona similis do not utilize prey exudates in the boundary layer, and instead rely on hydromechanical signals to detect prey . Cruise-swimming and current-generating copepods, such as C. helgolandicus, have been suggested to use remote chemical detection from the deformation of the boundary layer surrounding cells entrained in their feeding currents [39,40,41,42]. However, recent studies have questioned the theory of remote prey detection suggesting that prey exudates are only effective at much shorter distance [43,44]. Tiselius et al.  filmed encounters of two copepods (Pseudocalanus sp. and Paracalanus parvus) with various prey items, and determined that chemically-mediated remote sensing does not occur. In addition, by modeling the boundary layer chemical concentration, Tiselius et al.  conclude that remote sensing could only occur for “very large or unusually leaky cells”. It remains to be established if rapid volatile production, such as the liberation of DMS following microzooplankton grazing, constitutes sufficient chemical exudation allowing remote chemical detection by copepods.
At the meso- or patch scale, elevated background concentrations of infochemicals may stimulate an increase in foraging-related behaviors. This has been observed in copepods in response to amino acid additions (e.g., ) and cell-free prey filtrates . In contrast to the findings from earlier experiments using DMS microinjections into the flow-field of tethered T. longicornis , our experiments with C. helgolandicus suggested that grazing-mediated DMS has no clearly defined and consistent effect on swimming behavior when considering data pooled across all 12 individuals. C. helgolandicus spent approximately a quarter of the time budget dedicated to slow swimming in the low and high DMS treatments. This is consistent with the proportion of the time budget spent slow swimming by C. helgolandicus feeding on a range of dinoflagellate diets and in filtered seawater, whilst diatom prey resulted in an increase in swimming . The presence of Oxyrrhis marina in our experiments may provide a sufficient hydromechanical stimulus indicating the presence of a prey patch, potentially overriding the additional DMS cue in the high DMS treatment. Other studies have found copepod behavior to be closely coupled to prey density, with copepods in filtered seawater spending very little time slow swimming . Van Duren and Videler  demonstrated that the copepod Temora longicornis, a typical “cruise-swimmer” (in contrast to the “current-generating” behavioral strategy displayed by C. helgolandicus), modifies its swimming speed depending on prey density in accordance with predictions of optimal foraging theory. Woodson et al.  showed that copepods narrow their search area through a series of cue hierarchies. It is likely that the physical sensing of prey from hydromechanical cues or direct encounters provides copepods with cues for area-restricted search or feeding behaviors, whilst infochemicals provide a more general cue for copepods to display extended search behaviors . In a series of experiments designed to uncouple the relative importance of hydromechanical and chemical cues in copepod foraging behavior Buskey et al.  showed that the behavior of Pseudocalanus minutus differed depending on the cue received. In the presence of prey P. minutus decreased swimming speeds and increased turning rates, whereas cell-free prey exudates elicit an increase in swimming speed combined with a decrease in turning rate . Increasing the relative tortuosity of swimming trajectories within a prey patch would increase residency time allowing copepods to exploit the area of high productivity. Whilst increases in swimming speed and reductions in turning rate in response to prey cues, resulting in more ballistic swimming trajectories, would increase the rate of encountering a prey patch . It is possible that chemical cues such as DMS operate at larger scales than tested in our multitrophic interaction experiments, allowing C. helgolandicus to exploit directional cues to locate high productivity prey patches. For example, swarming behaviour in response to specific dissolved amino acids has been observed in laboratory experiments with the copepods A. hudsonica and Eurytemora herdmani . However, once a copepod encounters a high productivity prey patch hydromechanical cues appear to become the overriding foraging cue. Further experiments are required to determine the response of copepods to DMS and other bioactive compounds in heterogeneous chemical environments and to elucidate the interplay between prey density and infochemicals on feeding behavior.
We tested the hypothesis that grazing-induced DMS production following microzooplankton herbivory on phytoplankton would result in increased feeding behavior, measured in terms of time budget allocation towards slow swimming, in the copepod Calanus heloglandicus. Pooled data from 12 individual copepods over a 25-min period in treatments with either high or low grazing-induced DMS showed no difference in the total amount of the time budget allocated towards slow swimming.
Individual level responses showed a large degree of variation in between-individual behavior, both in terms of the total amount of time allocated towards slow swimming between copepods and in terms of the response of individual copepods to DMS treatments. Six of the twelve copepods showed a significant increase in slow swimming in low DMS treatments whereas only three copepods increased the proportion of slow swimming in high DMS treatments. This result highlights a cautionary note for assuming “average” copepod behavior and adds to the growing argument of the existence of individual behavioral “personalities” in invertebrates.
Our experiments replicate the scenario of a copepod encountering a high productivity prey patch with the addition of either high or low grazing-mediated DMS cues. In light of previous work and our results, we suggest that copepod behavioral responses to potential grazing-induced infochemicals, such as DMS, may act over larger spatio-temporal scales. Further work to determine the scale-dependent role of DMS as a chemical cue for copepods is required.
This study was supported through a Natural Environment Research Council grant (NERC–NE/H009485/1). The authors would like to thank John Stephens from the Plymouth Marine Laboratory for DMS quantification and Tania Cresswell-Maynard from the University of Essex for support with culturing. We thank J. Rudi Strickler from the Water Institute at the University of Wisconsin for helpful discussions and comments on the manuscript. We thank Susan Kimmance from the Plymouth Marine Laboratory and Stephen Archer from the Bigelow Laboratory for Ocean Sciences for advice during the preparation of the experiments.
Conflict of Interest
The authors declare no conflict of interest.
- Hay, M.E. Marine chemical ecology: Chemical signals and cues structure marine populations, communities, and ecosystems. Annu. Rev. Mar. Sci. 2009, 1, 193. [Google Scholar] [CrossRef]
- Ianora, A.; Bentley, M.G.; Caldwell, G.S.; Casotti, R.; Cembella, A.D.; Engström-Öst, J.; Halsband, C.; Sonnenschein, E.; Legrand, C.; Llewellyn, C.A. The relevance of marine chemical ecology to plankton and ecosystem function: An emerging field. Mar. Drugs 2011, 9, 1625–1648. [Google Scholar] [CrossRef]
- Amo, L.; Rodríguez-Gironés, M.Á.; Barbosa, A. Olfactory detection of dimethyl sulphide in a krill-eating Antarctic penguin. Mar. Ecol. Prog. Ser. 2013, 474, 277–285. [Google Scholar] [CrossRef]
- Cunningham, G.B.; Strauss, V.; Ryan, P.G. African penguins (Spheniscus demersus) can detect dimethyl sulphide, a prey-related odour. J. Exp. Biol. 2008, 211, 3123–3127. [Google Scholar] [CrossRef]
- Kowalewsky, S.; Dambach, M.; Mauck, B.; Dehnhardt, G. High olfactory sensitivity for dimethyl sulphide in harbour seals. Biol. Lett. 2006, 2, 106–109. [Google Scholar] [CrossRef]
- Nevitt, G.A.; Veit, R.R.; Kareiva, P. Dimethyl sulphide as a foraging cue for Antarctic procellariiform seabirds. Nature 1995, 376, 680–682. [Google Scholar] [CrossRef]
- Steinke, M.; Stefels, J.; Stamhuis, E. Dimethyl sulfide triggers search behavior in copepods. Limnol. Oceanogr. 2006, 51, 1925–1930. [Google Scholar] [CrossRef]
- Yang, E.J.; Kang, H.-K.; Yoo, S.; Hyun, J.-H. Contribution of auto-and heterotrophic protozoa to the diet of copepods in the Ulleung Basin, East Sea/Japan Sea. J. Plankton Res. 2009, 31, 647–659. [Google Scholar] [CrossRef]
- Fileman, E.; Petropavlovsky, A.; Harris, R. Grazing by the copepods Calanus helgolandicus and Acartia clausi on the protozooplankton community at station L4 in the Western English Channel. J. Plankton Res. 2010, 32, 709–724. [Google Scholar] [CrossRef]
- Lewis, N.D.; Breckels, M.N.; Archer, S.D.; Morozov, A.; Pitchford, J.W.; Steinke, M.; Codling, E.A. Grazing-induced production of DMS can stabilize food-web dynamics and promote the formation of phytoplankton blooms in a multitrophic plankton model. Biogeochemistry 2012, 110, 303–313. [Google Scholar] [CrossRef]
- Ferrer, R.P.; Zimmer, R.K. Molecules of keystone significance: Crucial agents in ecology and resource management. BioScience 2013, 63, 428–438. [Google Scholar] [CrossRef]
- Steinke, M.; Malin, G.; Liss, P.S. Trophic interactions in the sea: An ecological role for climate relevant volatiles? J. Phycol. 2002, 38, 630–638. [Google Scholar] [CrossRef]
- Widdicombe, C.E.; Eloire, D.; Harbour, D.; Harris, R.P.; Somerfield, P.J. Long-term phytoplankton community dynamics in the Western English Channel. J. Plankton Res. 2010, 32, 643–655. [Google Scholar] [CrossRef]
- Huskin, I.; Anadon, R.; Alvarez-Marques, F.; Harris, R.P. Ingestion, faecal pellet and egg production rates of Calanus helgolandicus feeding coccolithophorid vs. non-coccolithophorid diets. J. Exp. Mar. Biol. Ecol. 2000, 248, 239–254. [Google Scholar] [CrossRef]
- Hansen, F.C.; Reckermann, M.; Klein Breteler, W.C.; Riegman, R. Phaeocystis blooming enhanced by copepod predation on protozoa: Evidence from incubation experiments. Mar. Ecol. Prog. Ser. 1993, 102, 51–57. [Google Scholar] [CrossRef]
- Wolfe, G.V.; Steinke, M. Grazing-activated production of dimethyl sulfide (DMS) by two clones of Emiliania huxleyi. Limnol. Oceanogr. 1996, 41, 1151–1160. [Google Scholar] [CrossRef]
- Archer, S.D.; Stelfox-Widdicombe, C.E.; Burkill, P.H.; Malin, G. A dilution approach to quantify the production of dissolved dimethylsulphoniopropionate and dimethyl sulphide due to microzooplankton herbivory. Aquat. Microb. Ecol. 2001, 23, 131–145. [Google Scholar] [CrossRef]
- Costello, J.H.; Strickler, J.R.; Marrasé, C.; Trager, G.; Zeller, R.; Freise, A.J. Grazing in a turbulent environment: Behavioral response of a calanoid copepod, Centropages hamatus. Proc. Natl. Acad. Sci. USA 1990, 87, 1648–1652. [Google Scholar] [CrossRef]
- Hwang, J.-S.; Costello, J.H.; Strickler, J.R. Copepod grazing in turbulent flow: Elevated foraging behavior and habituation of escape responses. J. Plankton Res. 1994, 16, 421–431. [Google Scholar] [CrossRef]
- Cowles, T.J.; Strickler, J.R. Characterization of feeding activity patterns in the planktonic copepod Centropages typicus Kroyer under various food conditions. Limnol. Oceanogr. 1983, 28, 106–115. [Google Scholar] [CrossRef]
- Vlymen, W.J. Energy expenditure of swimming copepods. Limnol. Oceanogr. 1970, 15, 348–356. [Google Scholar] [CrossRef]
- Marrasé, C.; Costello, J.H.; Granata, T.; Strickler, J.R. Grazing in a turbulent environment: Energy dissipation, encounter rates, and efficacy of feeding currents in Centropages hamatus. Proc. Natl. Acad. Sci. USA 1990, 87, 1653–1657. [Google Scholar]
- Fields, D.; Yen, J. Outer limits and inner structure: The 3-dimensional flow field of Pleuromamma xiphias (Calanoida: Metridinidae). Bull. Mar. Sci. 1993, 53, 84–95. [Google Scholar]
- Van Duren, L.A.; Stamhuis, E.J.; Videler, J.J. Copepod feeding currents: Flow patterns, filtration rates and energetics. J. Exp. Biol. 2003, 206, 255–267. [Google Scholar] [CrossRef]
- Van Duren, L.A.; Videler, J.J. The trade-off between feeding, mate seeking and predator avoidance in copepods: Behavioural responses to chemical cues. J. Plankton Res. 1996, 18, 805–818. [Google Scholar] [CrossRef]
- Kiørboe, T. How zooplankton feed: Mechanisms, traits and trade-offs. Biol. Rev. 2011, 86, 311–339. [Google Scholar] [CrossRef]
- Hwang, J.-S.; Strickler, R. Can copepods differentiate prey from predator hydromechanically? Zool. Stud. 2001, 40, 1–6. [Google Scholar]
- Gill, C.W.; Harris, R.P. Behavioural responses of the copepods Calanus helgolandicus and Temora longicornis to dinoflagellate diets. J. Mar. Biol. Assoc. UK 1987, 67, 785–801. [Google Scholar] [CrossRef]
- Berges, J.A.; Franklin, D.J.; Harrison, P.J. Evolution of an artificial seawater medium: Improvements in enriched seawater, artificial water over the last two decades. J. Phycol. 2001, 37, 1138–1145. [Google Scholar] [CrossRef]
- Archer, S.D.; Cummings, D.G.; Llewellyn, C.A.; Fishwick, J.R. Phytoplankton taxa, irradiance and nutrient availability determine the seasonal cycle of DMSP in temperate shelf seas. Mar. Ecol. Prog. Ser. 2009, 394, 111–124. [Google Scholar] [CrossRef]
- R Development Core Team. R: A Language and Environment for Statistical Computing; Foundation for Statistical Computing: Vienna, Austria, 2012.
- Mendenhall, W.; Sincich, T. Statistics for Engineering and the Sciences, 5th ed.; Pearson Prentice-Hall: New Jersey, NJ, USA, 2007. [Google Scholar]
- Breckels, M.N. The Infochemical Role of Dimethyl Sulphide (DMS) and Related Compounds in Trophic Interactions. Ph.D. Thesis, The University of Essex, Colchester, UK, July 2010. [Google Scholar]
- Mather, J.A.; Logue, D.M. The Bold and the Spineless: Invertebrate Personalities. In Animal Personalities Behavior, Physiology, and Evolution; Carere, C., Maestripieri, D., Eds.; The University of Chicago Press: Chicago, IL, USA, 2013; pp. 13–35. [Google Scholar]
- Gherardi, F.; Aquiloni, L.; Tricarico, E. Behavioral plasticity, behavioral syndromes and animal personality in crustacean decapods: An imperfect map is better than no map. Curr. Zool. 2012, 58, 567–579. [Google Scholar]
- Dingemanse, N.J.; Wolf, M. Between-individual differences in behavioural plasticity within populations: Causes and consequences. Anim. Behav. 2012, 85, 1031–1039. [Google Scholar] [CrossRef]
- Woodson, C.B.; Webster, D.R.; Weissburg, M.J.; Yen, J. Cue hierarchy and foraging in calanoid copepods: Ecological implications of oceanographic structure. Mar. Ecol. Prog. Ser. 2007, 330, 163–177. [Google Scholar] [CrossRef]
- Svensen, C.; Kiørboe, T. Remote prey detection in Oithona similis: Hydromechanical versus chemical cues. J. Plankton Res. 2000, 22, 1155–1166. [Google Scholar] [CrossRef]
- Strickler, J.R. Calanoid copepods, feeding currents, and the role of gravity. Science 1982, 218, 158–160. [Google Scholar]
- Andrews, J.C. Deformation of the active space in the low Reynolds number feeding current of calanoid copepods. Can. J. Fish. Aquat. Sci. 1983, 40, 1293–1302. [Google Scholar] [CrossRef]
- Yen, J. Life in transition: Balancing inertial and viscous forces by planktonic copepods. Biol. Bull. 2000, 198, 213–224. [Google Scholar] [CrossRef]
- Jiang, H.; Osborn, T.R.; Meneveau, C. Chemoreception and the deformationof the active space in freely swimming copepods: A numerical study. J. Plankton Res. 2002, 24, 495–510. [Google Scholar] [CrossRef]
- Bruno, E.; Andersen Borg, C.M.; Kiørboe, T. Prey detection and prey capture in copepod nauplii. PLoS One 2012, 7, e47906. [Google Scholar]
- Tiselius, P.; Saiz, E.; Kiørboe, T. Sensory capabilities and food capture of two small copepods Paracalanus parvus and Pseudocalanus sp. Limnol. Oceanogr. 2013. [Google Scholar] [CrossRef]
- Poulet, S.A.; Gill, C.W. Spectral analyses of movements made by the cephalic appendages of copepods. Mar. Ecol. Prog. Ser. 1988, 43, 259–267. [Google Scholar] [CrossRef]
- Buskey, E.J. Swimming pattern as an indicator of the roles of copepod sensory systems in the recognition of food. Mar. Biol. 1984, 79, 165–175. [Google Scholar] [CrossRef]
- Visser, A.W.; Kiørboe, T. Plankton motility patterns and encounter rates. Oecologia 2006, 148, 538–546. [Google Scholar] [CrossRef]
- Poulet, S.A.; Ouellet, G. The role of amino acids in the chemosensory swarming and feeding of marine copepods. J. Plankton Res. 1982, 4, 341–361. [Google Scholar] [CrossRef]
© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).